Pyrene excimer-based dual-emission detection of a oligoaspartate tag-fused protein by using a ZnII-DpaTyr probe

Kei Honda; Sho Hei Fujishima; Akio Ojida; Itaru Hamachi

doi:10.1002/cbic.200700146

Pyrene excimer-based dual-emission detection of a oligoaspartate tag-fused protein by using a Zn^II-DpaTyr probe

Kei Honda, Sho Hei Fujishima, Akio Ojida, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

A dual-emission sensing system for proteins tethered to a repeated tetra-aspartate tag (D₄-tag) was successfully developed by using the binding-induced excimer formation of the pyrene-appended Zn^II-DpaTyr probe. This allows selective detection of the D₄-tag-fused protein in a simple and convenient manner by the naked eye. (Figure Presented)

Original language	English
Pages (from-to)	1370-1372
Number of pages	3
Journal	ChemBioChem
Volume	8
Issue number	12
DOIs	https://doi.org/10.1002/cbic.200700146
Publication status	Published - Aug 13 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200700146

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abstract = "A dual-emission sensing system for proteins tethered to a repeated tetra-aspartate tag (D4-tag) was successfully developed by using the binding-induced excimer formation of the pyrene-appended ZnII-DpaTyr probe. This allows selective detection of the D4-tag-fused protein in a simple and convenient manner by the naked eye. (Figure Presented)",

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AU - Hamachi, Itaru

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AB - A dual-emission sensing system for proteins tethered to a repeated tetra-aspartate tag (D4-tag) was successfully developed by using the binding-induced excimer formation of the pyrene-appended ZnII-DpaTyr probe. This allows selective detection of the D4-tag-fused protein in a simple and convenient manner by the naked eye. (Figure Presented)

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Pyrene excimer-based dual-emission detection of a oligoaspartate tag-fused protein by using a Zn^II-DpaTyr probe

Abstract

All Science Journal Classification (ASJC) codes

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