TY - JOUR
T1 - Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus
AU - Osamu, Chisaka
AU - Kimi, Araki
AU - Takahiro, Ochiya
AU - Toshiki, Tsurimoto
AU - Wacharee, Hiranyawasitte Attatippaholkun
AU - Noboru, Yanaihara
AU - Kenichi, Matsubara
N1 - Funding Information:
We thank Dr. T. Yura for providing us with E. coli strain KY 1603a nd invaluable advice. This work was supported in part by a grant-in-aid from the Science and Technology Agency of Japan.
PY - 1987
Y1 - 1987
N2 - A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.
AB - A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.
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U2 - 10.1016/0378-1119(87)90226-5
DO - 10.1016/0378-1119(87)90226-5
M3 - Article
C2 - 2832251
AN - SCOPUS:0023604821
SN - 0378-1119
VL - 60
SP - 183
EP - 189
JO - Gene
JF - Gene
IS - 2-3
ER -
