TY - JOUR
T1 - Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate form Candida rugosa
AU - Sakai, Kenji
AU - Tochikura, Tatsurokuro
AU - Takano, Koji
AU - Tachiki, Takashi
N1 - Funding Information:
Acknowledgment. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 570300037) from the Ministry of Education, Science and Culture of Japan.
PY - 1988
Y1 - 1988
N2 - A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220, 000) consisted of four identical subunits with molecular weight of 58, 000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.
AB - A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220, 000) consisted of four identical subunits with molecular weight of 58, 000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.
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U2 - 10.1271/bbb1961.52.2783
DO - 10.1271/bbb1961.52.2783
M3 - Article
AN - SCOPUS:85004448453
SN - 0002-1369
VL - 52
SP - 2783
EP - 2789
JO - Agricultural and Biological Chemistry
JF - Agricultural and Biological Chemistry
IS - 11
ER -