Purification and Properties of an Enzyme Oxidizing Nitrite to Nitrate form Candida rugosa

Kenji Sakai, Tatsurokuro Tochikura, Koji Takano, Takashi Tachiki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


A nitrite-oxidizing enzyme was isolated (1300-fold purification, 15% yield) from Candida rugosa IFO 0591 using a reaction mixture containing glucose oxidase and glucose. The enzyme (molecular weight 220, 000) consisted of four identical subunits with molecular weight of 58, 000. It showed absorption maxima at 277 and 405 nm with small peaks at 492, 535, and 625 nm. The spectrum was not altered by the addition of dithionite. These and other properties suggested that the enzyme was catalase and that the nitrite-oxidizing reaction was dependent on its peroxidase activity. Some aspects of the nitrite oxidation by the purified enzyme and various preparations of C. rugosa are described.

Original languageEnglish
Pages (from-to)2783-2789
Number of pages7
JournalAgricultural and Biological Chemistry
Issue number11
Publication statusPublished - 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


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