Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum

Soha G.R. Abdel-Salam, Masakazu Tsujikura, Masakazu Kondo, Tomonori Somamoto, Miki Nakao

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Zymosan, a yeast cell wall preparation that binds activated forms of complement C3, is a useful model target to activate the complement system. In our trial to analyze C3 diversity in Nile tilapia at the protein level using zymosan, we found that a novel 240-kDa serum protein (ZBP-240) also bound to zymosan in addition to C3-derived fragments. In the present study, we aimed to characterize tilapia C3 and ZBP-240, focusing on their immune-related functions. Four distinct C3 isoforms were purified from tilapia serum and shown to possess an intrachain thioester bond. ZBP-240 was also isolated from tilapia serum and examined for its binding properties to various microbial targets. As a result, ZBP-240 showed a wide spectrum of binding to Gram-positive and Gram-negative bacteria and yeasts. Amino acid sequence analysis of CNBr fragments of ZBP-240 suggested that this is a novel protein with no homologous sequence in protein databases. It was also suggested that the binding of ZBP-240 to microbes largely depends on hydrophobic interactions in a divalent-cation-independent manner, and that there may be a divalent-cation-dependent factor that enhances the binding of ZBP-240 in tilapia serum. Interestingly, ZBP-240 showed opsonic activity for tilapia kidney phagocytes at a level comparable to that of C3, implying that ZBP-240 is a novel teleost opsonic serum protein.

Original languageEnglish
Pages (from-to)301-310
Number of pages10
JournalFisheries science
Issue number2
Publication statusPublished - Mar 2014

All Science Journal Classification (ASJC) codes

  • Aquatic Science


Dive into the research topics of 'Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum'. Together they form a unique fingerprint.

Cite this