TY - JOUR
T1 - Purification and characterization of an oxygen-evolving photosystem II from Leptolyngbya sp. strain O-77
AU - Nakamori, Harutaka
AU - Yatabe, Takeshi
AU - Yoon, Ki Seok
AU - Ogo, Seiji
N1 - Funding Information:
This work was supported by the World Premier International Research Center Initiative (WPI), Grants-in-Aid: 23655053 , 25620047 , 25248017 , and 24109016 (Scientific Research on Innovative Areas “Stimuli-responsive Chemical Species”) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan , and the Basic Research Programs CREST Type, “Development of the Foundation for Nano-Interface Technology” from Japan Science and Technology Agency (JST) .
PY - 2014/8
Y1 - 2014/8
N2 - A new cyanobacterium of strain O-77 was isolated from a hot spring at Aso-Kuju National Park, Kumamoto, Japan. According to the phylogenetic analysis determined by 16S rRNA gene sequence, the strain O-77 belongs to the genus Leptolyngbya, classifying into filamentous non-heterocystous cyanobacteria. The strain O-77 showed the thermophilic behavior with optimal growth temperature of 55°C. Moreover, we have purified and characterized the oxygen-evolving photosystem II (PSII) from the strain O-77. The O2-evolving activity of the purified PSII from strain O-77 (PSIIO77) was 1275±255μmol O2 (mg Chl a)-1h-1. Based on the results of MALDI-TOF mass spectrometry and urea-SDS-PAGE analysis, the purified PSIIO77 was composite of the typical PSII components of CP47, CP43, PsbO, D2, D1, PsbV, PsbQ, PsbU, and several low molecular mass subunits. Visible absorption and 77K fluorescence spectra of the purified PSIIO77 were almost identical to those of other purified PSIIs from cyanobacteria. This report provides the successful example for the purification and characterization of an active PSII from thermophilic, filamentous non-heterocystous cyanobacteria.
AB - A new cyanobacterium of strain O-77 was isolated from a hot spring at Aso-Kuju National Park, Kumamoto, Japan. According to the phylogenetic analysis determined by 16S rRNA gene sequence, the strain O-77 belongs to the genus Leptolyngbya, classifying into filamentous non-heterocystous cyanobacteria. The strain O-77 showed the thermophilic behavior with optimal growth temperature of 55°C. Moreover, we have purified and characterized the oxygen-evolving photosystem II (PSII) from the strain O-77. The O2-evolving activity of the purified PSII from strain O-77 (PSIIO77) was 1275±255μmol O2 (mg Chl a)-1h-1. Based on the results of MALDI-TOF mass spectrometry and urea-SDS-PAGE analysis, the purified PSIIO77 was composite of the typical PSII components of CP47, CP43, PsbO, D2, D1, PsbV, PsbQ, PsbU, and several low molecular mass subunits. Visible absorption and 77K fluorescence spectra of the purified PSIIO77 were almost identical to those of other purified PSIIs from cyanobacteria. This report provides the successful example for the purification and characterization of an active PSII from thermophilic, filamentous non-heterocystous cyanobacteria.
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U2 - 10.1016/j.jbiosc.2014.01.009
DO - 10.1016/j.jbiosc.2014.01.009
M3 - Article
C2 - 24560665
AN - SCOPUS:84904401250
SN - 1389-1723
VL - 118
SP - 119
EP - 124
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 2
ER -