TY - JOUR
T1 - Purification and characterization of 4,5-dioxovalerate reductase (NADPH) from chlorella regularis
AU - Shioi, Yuzo
AU - Doi, Michio
AU - Sasa, Tsutomu
N1 - Funding Information:
This research was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1986/1
Y1 - 1986/1
N2 - Two types of 4,5-dioxovalerate reductases (NADPH) were partially purified and characterized from green alga, Chlorella regularis. The enzyme was separated by DEAE-Sephacel chromatography into two peaks: type I (first peak) and type II (second peak). The activity ratio of the type II to type I enzyme varied between 5 to 7 with a starting cell material. Both enzymes had the same pH optimum at 6.0 and pI value of 4.9. The molecular weight estimated by gel filtration was 33,000 for type I and 99,000 for type II enzyme. Both enzymes used only NADPH, but were not specific for 4,5-dioxovaleric acid (DOVA). Type I enzyme reduced glyoxylate 68-fold faster than DOVA, whereas type II enzyme acted more specifically on a variety of aldehydes than DOVA. It is suggested that these enzymes may not function primarily as NADPH-DOVA reductases in the metabolic pathway of DOVA.
AB - Two types of 4,5-dioxovalerate reductases (NADPH) were partially purified and characterized from green alga, Chlorella regularis. The enzyme was separated by DEAE-Sephacel chromatography into two peaks: type I (first peak) and type II (second peak). The activity ratio of the type II to type I enzyme varied between 5 to 7 with a starting cell material. Both enzymes had the same pH optimum at 6.0 and pI value of 4.9. The molecular weight estimated by gel filtration was 33,000 for type I and 99,000 for type II enzyme. Both enzymes used only NADPH, but were not specific for 4,5-dioxovaleric acid (DOVA). Type I enzyme reduced glyoxylate 68-fold faster than DOVA, whereas type II enzyme acted more specifically on a variety of aldehydes than DOVA. It is suggested that these enzymes may not function primarily as NADPH-DOVA reductases in the metabolic pathway of DOVA.
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M3 - Article
AN - SCOPUS:77957176649
SN - 0032-0781
VL - 27
SP - 67
EP - 74
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 1
ER -