PUFAs in serum cholesterol ester and oxidative DNA damage in Japanese men and women

Yasumi Kimura, Masao Sato, Kayo Kurotani, Akiko Nanri, Kazuaki Kawai, Hiroshi Kasai, Katsumi Imaizumi, Tetsuya Mizoue

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Background: PUFAs are susceptible to lipid peroxidation and play a role in inflammation, both of which can induce oxidative stress. However, the relation of PUFA to oxidative DNA damage in humans is elusive. Objective: We examined the association between concentrations of circulatory PUFAs and urinary 8-oxo-7,8-dihydroguanine (8-oxoGua) in Japanese men and women. Design: The subjects were 495 participants (290 men and 205 women) in a cross-sectional study in 2 municipal offices in Japan. Serum cholesterol ester (CE) and phospholipid fatty acid composition were measured by gas-liquid chromatography. Urinary 8-oxoGua concentrations were measured by HPLC, and 8-oxoGua values for each tertile of PUFA after adjustment for covariates were calculated by multiple regression. Results: Urinary 8-oxoGua concentrations increased with increasing concentrations of n-3 (omega-3) PUFAs, EPA, and DHA in serum CE (P-trend = 0.001, 0.01, and 0.009, respectively), whereas they decreased with increasing concentrations of n-6 PUFAs and linoleic acid (P-trend = 0.02 and 0.051, respectively). Conclusion: Oxidative DNA damage may be greater with higher concentrations of long-chain n-3 PUFAs but lower with higher concentrations of n-6 (omega-6) PUFAs.

Original languageEnglish
Pages (from-to)1209-1214
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Issue number5
Publication statusPublished - May 1 2012

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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