TY - JOUR
T1 - PTEN/MMAC1 mutations identified in small cell, but not in non-small cell lung cancers
AU - Yokomizo, Akira
AU - Tindall, Donald J.
AU - Drabkin, Harry
AU - Gemmill, Robert
AU - Franklin, Wilbur
AU - Yang, Ping
AU - Sugio, Kenji
AU - Smith, David I.
AU - Liu, Wanguo
N1 - Funding Information:
This work was supported by NIH Grant CA48031 to DIS, CA58187 to HD, RG, WF and by the Mayo Foundation.
PY - 1998/7/30
Y1 - 1998/7/30
N2 - A putative tumor suppressor, PTEN/MMAC1 gene at 10q23 was recently identified and found to be mutated in many different human tumors. To determine the role of the PTEN/MMAC1 gene in lung cancer, we screened 34 small cell lung cancer (SCLC) cell lines, 10 SCLC tumors, 13 non-small cell lung cancer (NSCLC) cell lines and 10 NSCLC tumors using Denaturing HPLC (DHPLC) and direct sequencing methods. In SCLC, six (18%) of the cell lines and one of the primary tumor samples (10%) showed alterations of the PTEN/MMAC1 gene including point mutations, small fragment deletions, and homozygous deletions. All of the point mutations and small fragment deletions were observed in hemizygously deleted cell lines. In contrast to SCLC, none of the NSCLC tumors or cell lines had mutations in the PTEN/MMAC1 gene. These data indicate that PTEN/MMAC1 mutations contribute to the pathogenesis and neoplastic evolution in SCLC but not in NSCLC.
AB - A putative tumor suppressor, PTEN/MMAC1 gene at 10q23 was recently identified and found to be mutated in many different human tumors. To determine the role of the PTEN/MMAC1 gene in lung cancer, we screened 34 small cell lung cancer (SCLC) cell lines, 10 SCLC tumors, 13 non-small cell lung cancer (NSCLC) cell lines and 10 NSCLC tumors using Denaturing HPLC (DHPLC) and direct sequencing methods. In SCLC, six (18%) of the cell lines and one of the primary tumor samples (10%) showed alterations of the PTEN/MMAC1 gene including point mutations, small fragment deletions, and homozygous deletions. All of the point mutations and small fragment deletions were observed in hemizygously deleted cell lines. In contrast to SCLC, none of the NSCLC tumors or cell lines had mutations in the PTEN/MMAC1 gene. These data indicate that PTEN/MMAC1 mutations contribute to the pathogenesis and neoplastic evolution in SCLC but not in NSCLC.
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U2 - 10.1038/sj.onc.1201956
DO - 10.1038/sj.onc.1201956
M3 - Article
C2 - 9696041
AN - SCOPUS:0032581519
SN - 0950-9232
VL - 17
SP - 475
EP - 479
JO - Oncogene
JF - Oncogene
IS - 4
ER -
