TY - JOUR
T1 - Pseudo-Membrane Jackets
T2 - Two-Dimensional Coordination Polymers Achieving Visible Phase Separation in Cell Membrane
AU - Ohtani, Ryo
AU - Kawano, Kenichi
AU - Kinoshita, Masanao
AU - Yanaka, Saeko
AU - Watanabe, Hikaru
AU - Hirai, Kenji
AU - Futaki, Shiroh
AU - Matsumori, Nobuaki
AU - Uji-i, Hiroshi
AU - Ohba, Masaaki
AU - Kato, Koichi
AU - Hayami, Shinya
N1 - Funding Information:
This work was supported by JSPS Grant‐in‐Aid for Young Scientists JP18K14245 (R.O.) and JP18K14664 (K.Kawano), JSPS Grant‐in‐Aid for Scientific Research on Innovative Areas (Dynamical Ordering & Integrated Functions JP16H00777, JP25102008, JP25102001, JP15K21708, Mixed Anion JP19H04701). This work was also supported by KAKENHI Grant‐in‐Aid for Scientific Research (A) JP17H01200 and (B) JP17H03003. This work was partially supported by the Cooperative Research Program of “Network Joint Research Centre for Materials and Devices” and Kyushu Synchrotron Light Research Center 1809078F and 1807056F. This research was supported by Joint Research by Institute for Molecular Science (IMS). (IMS program No.101, 102, and 20‐101)
Funding Information:
This work was supported by JSPS Grant-in-Aid for Young Scientists JP18K14245 (R.O.) and JP18K14664 (K.Kawano), JSPS Grant-in-Aid for Scientific Research on Innovative Areas (Dynamical Ordering & Integrated Functions JP16H00777, JP25102008, JP25102001, JP15K21708, Mixed Anion JP19H04701). This work was also supported by KAKENHI Grant-in-Aid for Scientific Research (A) JP17H01200 and (B) JP17H03003. This work was partially supported by the Cooperative Research Program of ?Network Joint Research Centre for Materials and Devices? and Kyushu Synchrotron Light Research Center 1809078F and 1807056F. This research was supported by Joint Research by Institute for Molecular Science (IMS). (IMS program No.101, 102, and 20-101)
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/10/5
Y1 - 2020/10/5
N2 - Cell membranes contain lateral systems that consist of various lipid compositions and actin cytoskeleton, providing two-dimensional (2D) platforms for chemical reactions. However, such complex 2D environments have not yet been used as a synthetic platform for artificial 2D nanomaterials. Herein, we demonstrate the direct synthesis of 2D coordination polymers (CPs) at the liquid-cell interface of the plasma membrane of living cells. The coordination-driven self-assembly of networking metal complex lipids produces cyanide-bridged CP layers with metal ions, enabling “pseudo-membrane jackets” that produce long-lived micro-domains with a size of 1–5 μm. The resultant artificial and visible phase separation systems remain stable even in the absence of actin skeletons in cells. Moreover, we show the cell application of the jackets by demonstrating the enhancement of cellular calcium response to ATP.
AB - Cell membranes contain lateral systems that consist of various lipid compositions and actin cytoskeleton, providing two-dimensional (2D) platforms for chemical reactions. However, such complex 2D environments have not yet been used as a synthetic platform for artificial 2D nanomaterials. Herein, we demonstrate the direct synthesis of 2D coordination polymers (CPs) at the liquid-cell interface of the plasma membrane of living cells. The coordination-driven self-assembly of networking metal complex lipids produces cyanide-bridged CP layers with metal ions, enabling “pseudo-membrane jackets” that produce long-lived micro-domains with a size of 1–5 μm. The resultant artificial and visible phase separation systems remain stable even in the absence of actin skeletons in cells. Moreover, we show the cell application of the jackets by demonstrating the enhancement of cellular calcium response to ATP.
UR - http://www.scopus.com/inward/record.url?scp=85089363213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85089363213&partnerID=8YFLogxK
U2 - 10.1002/anie.202006600
DO - 10.1002/anie.202006600
M3 - Article
C2 - 32608036
AN - SCOPUS:85089363213
SN - 1433-7851
VL - 59
SP - 17931
EP - 17937
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 41
ER -