Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells

Soichiro Sonoda, Haruyoshi Yamaza, Koichiro Yoshimaru, Tomoaki Taguchi, Takayoshi Yamaza

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).

Original languageEnglish
Article number101386
JournalSTAR Protocols
Issue number2
Publication statusPublished - Jun 17 2022

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology
  • General Neuroscience
  • General Medicine


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