Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells

Yoshinori Katakura; Miyako Udono; Kazuyuki Katsuki; Hisaya Nishide; Yukiko Tabira; Takahiro Ikei; Makiko Yamashita; Tsukasa Fujiki; Sanetaka Shirahata

doi:10.1093/jb/mvp046

Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells

Yoshinori Katakura, Miyako Udono, Kazuyuki Katsuki, Hisaya Nishide, Yukiko Tabira, Takahiro Ikei, Makiko Yamashita, Tsukasa Fujiki, Sanetaka Shirahata

Systems Biology

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19 Citations (Scopus)

Abstract

In the present study, we clarified that transforming growth factor β (TGF-β) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-β-induced cellular senescence. Among PKCs, we showed that PKC-δ induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-δ in cellular senescence programs was demonstrated using a kinase negative PKC-δ and small interfering RNA against PKC-δ. Furthermore, PKC-δ was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-δ plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-δ.

Original language	English
Pages (from-to)	87-93
Number of pages	7
Journal	Journal of biochemistry
Volume	146
Issue number	1
DOIs	https://doi.org/10.1093/jb/mvp046
Publication status	Published - Jul 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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title = "Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells",

abstract = "In the present study, we clarified that transforming growth factor β (TGF-β) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-β-induced cellular senescence. Among PKCs, we showed that PKC-δ induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-δ in cellular senescence programs was demonstrated using a kinase negative PKC-δ and small interfering RNA against PKC-δ. Furthermore, PKC-δ was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-δ plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-δ.",

author = "Yoshinori Katakura and Miyako Udono and Kazuyuki Katsuki and Hisaya Nishide and Yukiko Tabira and Takahiro Ikei and Makiko Yamashita and Tsukasa Fujiki and Sanetaka Shirahata",

note = "Funding Information: Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.",

year = "2009",

month = jul,

doi = "10.1093/jb/mvp046",

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pages = "87--93",

journal = "Journal of biochemistry",

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T1 - Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells

AU - Katakura, Yoshinori

AU - Udono, Miyako

AU - Katsuki, Kazuyuki

AU - Nishide, Hisaya

AU - Tabira, Yukiko

AU - Ikei, Takahiro

AU - Yamashita, Makiko

AU - Fujiki, Tsukasa

AU - Shirahata, Sanetaka

N1 - Funding Information: Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.

PY - 2009/7

Y1 - 2009/7

N2 - In the present study, we clarified that transforming growth factor β (TGF-β) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-β-induced cellular senescence. Among PKCs, we showed that PKC-δ induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-δ in cellular senescence programs was demonstrated using a kinase negative PKC-δ and small interfering RNA against PKC-δ. Furthermore, PKC-δ was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-δ plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-δ.

AB - In the present study, we clarified that transforming growth factor β (TGF-β) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-β-induced cellular senescence. Among PKCs, we showed that PKC-δ induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-δ in cellular senescence programs was demonstrated using a kinase negative PKC-δ and small interfering RNA against PKC-δ. Furthermore, PKC-δ was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-δ plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-δ.

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JO - Journal of biochemistry

JF - Journal of biochemistry

IS - 1

ER -

Protein kinase C δ plays a key role in cellular senescence programs of human normal diploid cells

Abstract

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