Protein extraction by reversed MiCelles using new surfactants

M. Goto, K. Kondo, F. Nakashio

    Research output: Chapter in Book/Report/Conference proceedingChapter

    4 Citations (Scopus)


    A surfactant plays a key role in the protein extraction by reversed micelles. In this study, extraction of some proteins by new reversed micelle was carried out using dioleyl phosphoric acid (abbreviated DOLPA) instead of conventional surfactant AOT. Large protein more than 50000 of molecular weight, which is difficult to dissolve into AOT reversed micelle, could be easily extracted by DOLPA reversed micelle. Denaturation of the proteins was depressed by using DOLPA reversed micelle because of the poor solubility of the DOLPA to the aqueous solution. Further, hybrid reversed micelle of DOLPA and AOT was investigated. It is found that extracted ratio of a - chymotrypsin was increased by use of the hybrid reversed micelle.

    Original languageEnglish
    Title of host publicationProcess Metallurgy
    Number of pages6
    Publication statusPublished - Jan 1 1992

    Publication series

    NameProcess Metallurgy
    ISSN (Print)1572-4409

    All Science Journal Classification (ASJC) codes

    • Geotechnical Engineering and Engineering Geology
    • Industrial and Manufacturing Engineering


    Dive into the research topics of 'Protein extraction by reversed MiCelles using new surfactants'. Together they form a unique fingerprint.

    Cite this