Abstract
Protein extraction was performed by a calix[6]arene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calix[6]arene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.
Original language | English |
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Pages (from-to) | 185-189 |
Number of pages | 5 |
Journal | Solvent Extraction Research and Development |
Volume | 10 |
Publication status | Published - 2003 |
All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Chemical Engineering(all)