Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles

Akiho Hirao; Naotomo Tottori; Maasa Yokomori; Miho Tagawa; Shigeo S. Sugano; Shinya Sakuma; Yoko Yamanishi

doi:10.1109/MEMS51782.2021.9375180

Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles

Akiho Hirao, Naotomo Tottori, Maasa Yokomori, Miho Tagawa, Shigeo S. Sugano, Shinya Sakuma, Yoko Yamanishi

Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present a novel concept of microfluidic system that enable promoting the protein crystal nucleation, monitoring the protein crystallization state, and analysis the crystal structure in a sequential process. This proposed system comprised of four main functional parts: (i) the protein crystal nucleation using an electrically induced microbubble injector, (ii) droplets generation by microchannel, (iii) monitoring of a crystal morphology in droplets to optimize their incubation time, (iv) sorting of droplets containing produced crystals suitable for X-ray crystallography and analyzing the protein structure. As the first step of this research, in this study, we developed a critical functional part of the concept that enable control of the protein crystallization and could confirm the mean number of protein crystal in droplets, which was treated by electrically induced bubble, increased approximately 1.5 times as compared with that of protein crystal in untreated droplets.

Original language	English
Title of host publication	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	168-171
Number of pages	4
ISBN (Electronic)	9781665419123
DOIs	https://doi.org/10.1109/MEMS51782.2021.9375180
Publication status	Published - Jan 25 2021
Event	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States Duration: Jan 25 2021 → Jan 29 2021

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2021-January
ISSN (Print)	1084-6999

Conference

Conference	34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
Country/Territory	United States
City	Virtual, Gainesville
Period	1/25/21 → 1/29/21

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS51782.2021.9375180

Cite this

Hirao, A., Tottori, N., Yokomori, M., Tagawa, M., Sugano, S. S., Sakuma, S., & Yamanishi, Y. (2021). Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles. In 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 (pp. 168-171). Article 9375180 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS51782.2021.9375180

Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles. / Hirao, Akiho; Tottori, Naotomo ; Yokomori, Maasa et al.
34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. p. 168-171 9375180 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2021-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hirao, A, Tottori, N , Yokomori, M, Tagawa, M, Sugano, SS, Sakuma, S & Yamanishi, Y 2021, Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles. in 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021., 9375180, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2021-January, Institute of Electrical and Electronics Engineers Inc., pp. 168-171, 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021, Virtual, Gainesville, United States, 1/25/21. https://doi.org/10.1109/MEMS51782.2021.9375180

Hirao A, Tottori N , Yokomori M, Tagawa M, Sugano SS, Sakuma S et al. Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles. In 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc. 2021. p. 168-171. 9375180. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS51782.2021.9375180

Hirao, Akiho ; Tottori, Naotomo ; Yokomori, Maasa et al. / Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles. 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021. Institute of Electrical and Electronics Engineers Inc., 2021. pp. 168-171 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "We present a novel concept of microfluidic system that enable promoting the protein crystal nucleation, monitoring the protein crystallization state, and analysis the crystal structure in a sequential process. This proposed system comprised of four main functional parts: (i) the protein crystal nucleation using an electrically induced microbubble injector, (ii) droplets generation by microchannel, (iii) monitoring of a crystal morphology in droplets to optimize their incubation time, (iv) sorting of droplets containing produced crystals suitable for X-ray crystallography and analyzing the protein structure. As the first step of this research, in this study, we developed a critical functional part of the concept that enable control of the protein crystallization and could confirm the mean number of protein crystal in droplets, which was treated by electrically induced bubble, increased approximately 1.5 times as compared with that of protein crystal in untreated droplets.",

author = "Akiho Hirao and Naotomo Tottori and Maasa Yokomori and Miho Tagawa and Sugano, {Shigeo S.} and Shinya Sakuma and Yoko Yamanishi",

note = "Funding Information: This work was supported by JST CREST Grant No JPMJCR 19S6. Publisher Copyright: {\textcopyright} 2021 IEEE.; 34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 ; Conference date: 25-01-2021 Through 29-01-2021",

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AU - Sakuma, Shinya

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AB - We present a novel concept of microfluidic system that enable promoting the protein crystal nucleation, monitoring the protein crystallization state, and analysis the crystal structure in a sequential process. This proposed system comprised of four main functional parts: (i) the protein crystal nucleation using an electrically induced microbubble injector, (ii) droplets generation by microchannel, (iii) monitoring of a crystal morphology in droplets to optimize their incubation time, (iv) sorting of droplets containing produced crystals suitable for X-ray crystallography and analyzing the protein structure. As the first step of this research, in this study, we developed a critical functional part of the concept that enable control of the protein crystallization and could confirm the mean number of protein crystal in droplets, which was treated by electrically induced bubble, increased approximately 1.5 times as compared with that of protein crystal in untreated droplets.

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Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles

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