Protective effects of lotus stamen extract on uv-b-irradiated skin cells activities

Preeyawass Phimnuan, Racharat Rukchay, Nichanan Tiraravesit, Sombat Wanauppathamkul, Suppisak Chaturonratsamee, Prapadsorn Pittayanurak, Vichai Reutrakul, Jarupa Viyoch

Research output: Contribution to journalArticlepeer-review


Traditionally, Nelumbo nucifera has been used as a tonic and stimulant. The aim of this study was to clarify a protective effect regarding UV-B induced inflammation and melanogenesis activities of the crude extract of the dried lotus stamen extract. The compounds of the extract were isolated by column chromatography. The anti-oxidant EC50 value of the crude extract was 0.0353 mg/mL while the EC50 values of the isolated compounds of kaempferol and kaempferol 3-O-β-D-glucopyranoside were 0.0030 and 0.0077 mg/mL, respectively, which were observed by DPPH assay. The tyrosinase inhibitory activity was tested by tyrosinase enzyme. The anti-tyrosinase IC50 value of the crude was 0.0192 mg/mL while the isolated kaempferol was 0.0496 mg/mL. To determine the anti-inflammatory activity, the molecular targets for the extract action were TNF-α and IL-6. The release of TNF-α and IL-6 was significantly reduced in UV-B-induced keratinocytes pretreated with the crude extract at concentrations from 25 to 100 μg/mL. Moreover, the crude extract was found to inhibit the melanin overproduction of B16F1 induced by α-MSH.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalSongklanakarin Journal of Science and Technology
Issue number2
Publication statusPublished - Mar 1 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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