TY - JOUR
T1 - Protective effects of lotus stamen extract on uv-b-irradiated skin cells activities
AU - Phimnuan, Preeyawass
AU - Rukchay, Racharat
AU - Tiraravesit, Nichanan
AU - Wanauppathamkul, Sombat
AU - Chaturonratsamee, Suppisak
AU - Pittayanurak, Prapadsorn
AU - Reutrakul, Vichai
AU - Viyoch, Jarupa
N1 - Funding Information:
We thank the grant from Naresuan University for the financial support of the research and the International Laboratories Corp. Ltd., Thailand for providing the lotus stamen extract. We also thank the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Office of the Higher Education Commission, Thailand for the use of their facilities.
Publisher Copyright:
© (Publication Year), (publisher Name). All rights reserved.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Traditionally, Nelumbo nucifera has been used as a tonic and stimulant. The aim of this study was to clarify a protective effect regarding UV-B induced inflammation and melanogenesis activities of the crude extract of the dried lotus stamen extract. The compounds of the extract were isolated by column chromatography. The anti-oxidant EC50 value of the crude extract was 0.0353 mg/mL while the EC50 values of the isolated compounds of kaempferol and kaempferol 3-O-β-D-glucopyranoside were 0.0030 and 0.0077 mg/mL, respectively, which were observed by DPPH assay. The tyrosinase inhibitory activity was tested by tyrosinase enzyme. The anti-tyrosinase IC50 value of the crude was 0.0192 mg/mL while the isolated kaempferol was 0.0496 mg/mL. To determine the anti-inflammatory activity, the molecular targets for the extract action were TNF-α and IL-6. The release of TNF-α and IL-6 was significantly reduced in UV-B-induced keratinocytes pretreated with the crude extract at concentrations from 25 to 100 μg/mL. Moreover, the crude extract was found to inhibit the melanin overproduction of B16F1 induced by α-MSH.
AB - Traditionally, Nelumbo nucifera has been used as a tonic and stimulant. The aim of this study was to clarify a protective effect regarding UV-B induced inflammation and melanogenesis activities of the crude extract of the dried lotus stamen extract. The compounds of the extract were isolated by column chromatography. The anti-oxidant EC50 value of the crude extract was 0.0353 mg/mL while the EC50 values of the isolated compounds of kaempferol and kaempferol 3-O-β-D-glucopyranoside were 0.0030 and 0.0077 mg/mL, respectively, which were observed by DPPH assay. The tyrosinase inhibitory activity was tested by tyrosinase enzyme. The anti-tyrosinase IC50 value of the crude was 0.0192 mg/mL while the isolated kaempferol was 0.0496 mg/mL. To determine the anti-inflammatory activity, the molecular targets for the extract action were TNF-α and IL-6. The release of TNF-α and IL-6 was significantly reduced in UV-B-induced keratinocytes pretreated with the crude extract at concentrations from 25 to 100 μg/mL. Moreover, the crude extract was found to inhibit the melanin overproduction of B16F1 induced by α-MSH.
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U2 - 10.14456/sjst-psu.2020.50
DO - 10.14456/sjst-psu.2020.50
M3 - Article
AN - SCOPUS:85084083699
SN - 0125-3395
VL - 42
SP - 383
EP - 390
JO - Songklanakarin Journal of Science and Technology
JF - Songklanakarin Journal of Science and Technology
IS - 2
ER -