Protective Effect of κ-Carrageenan against Bacterial Infections in Carp Cyprinus carpio

Kazuhiro Fujiki; Dong Ho Shin; Miki Nakao; Tomoki Yano

doi:10.5109/24198

Protective Effect of κ-Carrageenan against Bacterial Infections in Carp Cyprinus carpio

Kazuhiro Fujiki, Dong Ho Shin, Miki Nakao, Tomoki Yano

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A hot-water extract of tsunomata Chondrus ocellatus was found to contain polysaccharide(s) which showed immunoenhancing activity in fish. In order to identify the effective component, the hot-water extract of tsunomata was fractionated and 0.3 M KCl-insoluble and -soluble fractions (Fractions I and II) were evaluated for their potential to enhance protection against Edwardsiella tarda and Aeromonas hydrophila infections in carp. Intraperitoneal injection of Fraction I (2-3 mg/100g body weight) 6 and 3 days prior to challenge with the pathogenic bacteria resulted in significantly greater survival than that of the control fish. On the other hand, Fraction II was not effective against the pathogenic bacteria at any dose. Chemical and physicochemical analyses revealed that Fraction I was κ-carrageenan containing a trace quantity of ι-carrageenan.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Journal of the Faculty of Agriculture, Kyushu University
Volume	42
Issue number	1-2
DOIs	https://doi.org/10.5109/24198
Publication status	Published - Dec 1997

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Biotechnology

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title = "Protective Effect of κ-Carrageenan against Bacterial Infections in Carp Cyprinus carpio",

abstract = "A hot-water extract of tsunomata Chondrus ocellatus was found to contain polysaccharide(s) which showed immunoenhancing activity in fish. In order to identify the effective component, the hot-water extract of tsunomata was fractionated and 0.3 M KCl-insoluble and -soluble fractions (Fractions I and II) were evaluated for their potential to enhance protection against Edwardsiella tarda and Aeromonas hydrophila infections in carp. Intraperitoneal injection of Fraction I (2-3 mg/100g body weight) 6 and 3 days prior to challenge with the pathogenic bacteria resulted in significantly greater survival than that of the control fish. On the other hand, Fraction II was not effective against the pathogenic bacteria at any dose. Chemical and physicochemical analyses revealed that Fraction I was κ-carrageenan containing a trace quantity of ι-carrageenan.",

author = "Kazuhiro Fujiki and Shin, {Dong Ho} and Miki Nakao and Tomoki Yano",

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T1 - Protective Effect of κ-Carrageenan against Bacterial Infections in Carp Cyprinus carpio

AU - Fujiki, Kazuhiro

AU - Shin, Dong Ho

AU - Nakao, Miki

AU - Yano, Tomoki

PY - 1997/12

Y1 - 1997/12

N2 - A hot-water extract of tsunomata Chondrus ocellatus was found to contain polysaccharide(s) which showed immunoenhancing activity in fish. In order to identify the effective component, the hot-water extract of tsunomata was fractionated and 0.3 M KCl-insoluble and -soluble fractions (Fractions I and II) were evaluated for their potential to enhance protection against Edwardsiella tarda and Aeromonas hydrophila infections in carp. Intraperitoneal injection of Fraction I (2-3 mg/100g body weight) 6 and 3 days prior to challenge with the pathogenic bacteria resulted in significantly greater survival than that of the control fish. On the other hand, Fraction II was not effective against the pathogenic bacteria at any dose. Chemical and physicochemical analyses revealed that Fraction I was κ-carrageenan containing a trace quantity of ι-carrageenan.

AB - A hot-water extract of tsunomata Chondrus ocellatus was found to contain polysaccharide(s) which showed immunoenhancing activity in fish. In order to identify the effective component, the hot-water extract of tsunomata was fractionated and 0.3 M KCl-insoluble and -soluble fractions (Fractions I and II) were evaluated for their potential to enhance protection against Edwardsiella tarda and Aeromonas hydrophila infections in carp. Intraperitoneal injection of Fraction I (2-3 mg/100g body weight) 6 and 3 days prior to challenge with the pathogenic bacteria resulted in significantly greater survival than that of the control fish. On the other hand, Fraction II was not effective against the pathogenic bacteria at any dose. Chemical and physicochemical analyses revealed that Fraction I was κ-carrageenan containing a trace quantity of ι-carrageenan.

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Protective Effect of κ-Carrageenan against Bacterial Infections in Carp Cyprinus carpio

Abstract

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