TY - JOUR
T1 - Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins
AU - Ishida, Takumi
AU - Kawakami, Masayo
AU - Baba, Hiroko
AU - Yahata, Masahiro
AU - Mutoh, Junpei
AU - Takeda, Shuso
AU - Fujita, Hideaki
AU - Tanaka, Yoshitaka
AU - Ishii, Yuji
AU - Yamada, Hideyuki
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for Young Scientists (B) (no. 18790100) from the Japan Society for the Promotion of Science. This work was also supported in part by a grant (research no. 15190101) from the Ministry of Health, Labour and Welfare of Japan, and a Grant-in-Aid for Scientific Research on Priority Areas (no. 18059026) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2008/11
Y1 - 2008/11
N2 - The effect of proteasome inhibition with N-acetyl-leucyl-leucyl-norleucinal (ALLN) on the protein expression regulated by aryl hydrocarbon receptor (AhR) was studied in T47D breast tumor cells. The luciferase reporter gene assay using a construct which has the xenobiotic responsive element showed that the inducible expression of the reporter with AhR ligands was significantly reduced by co-treatment with ALLN. The same suppressive effect by ALLN was observed for ethoxyresorufin O-deethylase (EROD) activity induced by an AhR ligand, 3-methylcholanthrene (3MC). Despite the above effects, the induced expression of CYP1A1 and CYP1B1 mRNAs was unaffected by ALLN. While lactacystin, another proteasome inhibitor, exhibited the same effect as ALLN on EROD activity induced by 3MC, leupeptin, which is one of the cysteine protease inhibitors, had no such effect. Based on the evidence obtained, it appears that proteasome inhibition results in a reduction in the expression of AhR-regulated proteins.
AB - The effect of proteasome inhibition with N-acetyl-leucyl-leucyl-norleucinal (ALLN) on the protein expression regulated by aryl hydrocarbon receptor (AhR) was studied in T47D breast tumor cells. The luciferase reporter gene assay using a construct which has the xenobiotic responsive element showed that the inducible expression of the reporter with AhR ligands was significantly reduced by co-treatment with ALLN. The same suppressive effect by ALLN was observed for ethoxyresorufin O-deethylase (EROD) activity induced by an AhR ligand, 3-methylcholanthrene (3MC). Despite the above effects, the induced expression of CYP1A1 and CYP1B1 mRNAs was unaffected by ALLN. While lactacystin, another proteasome inhibitor, exhibited the same effect as ALLN on EROD activity induced by 3MC, leupeptin, which is one of the cysteine protease inhibitors, had no such effect. Based on the evidence obtained, it appears that proteasome inhibition results in a reduction in the expression of AhR-regulated proteins.
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U2 - 10.1016/j.etap.2008.07.004
DO - 10.1016/j.etap.2008.07.004
M3 - Article
C2 - 21791387
AN - SCOPUS:52949142363
SN - 1382-6689
VL - 26
SP - 348
EP - 354
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 3
ER -