TY - JOUR
T1 - Property of IK,n in inner hair cells isolated from guinea-pig cochlea
AU - Kimitsuki, Takashi
AU - Komune, Noritaka
AU - Noda, Teppei
AU - Takaiwa, Kazutaka
AU - Ohashi, Mitsuru
AU - Komune, Shizuo
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research 9591978 , 21592160 from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Research Fund of Institute of Kampo Medicine (Japan).
PY - 2010/3
Y1 - 2010/3
N2 - One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.
AB - One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.
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U2 - 10.1016/j.heares.2010.01.002
DO - 10.1016/j.heares.2010.01.002
M3 - Article
C2 - 20060884
AN - SCOPUS:76949084941
SN - 0378-5955
VL - 261
SP - 57
EP - 62
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -