TY - JOUR
T1 - Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae
AU - Oba, Takahiro
AU - Yamamoto, Yoshitsugu
AU - Nomiyama, Shuji
AU - Suenaga, Hikaru
AU - Muta, Shigeru
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
N1 - Funding Information:
This research was partially supported by the Research for the Future Program of JSPS, and by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Ms. Kyoko Ueda for amino acid analysis.
PY - 2006
Y1 - 2006
N2 - We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
AB - We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
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U2 - 10.1271/bbb.50640
DO - 10.1271/bbb.50640
M3 - Article
C2 - 16861814
AN - SCOPUS:33746470236
SN - 0916-8451
VL - 70
SP - 1776
EP - 1779
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -