Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae

Takahiro Oba, Yoshitsugu Yamamoto, Shuji Nomiyama, Hikaru Suenaga, Shigeru Muta, Kosuke Tashiro, Satoru Kuhara

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.

Original languageEnglish
Pages (from-to)1776-1779
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number7
Publication statusPublished - 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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