TY - JOUR
T1 - Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe
AU - Matsuzawa, Tomohiko
AU - Hara, Futoshi
AU - Tohda, Hideki
AU - Uemura, Hiroshi
AU - Takegawa, Kaoru
N1 - Funding Information:
Acknowledgments We thank Dr. Hirofumi Aiba for S. pombe strains. This work was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. T.M. was supported in part by Japan Society for the Promotion of Science (JSPS).
PY - 2012/7
Y1 - 2012/7
N2 - The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1 + and fbp1 + during glucose starvation even though gld1 + and fbp1 + are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1 + was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1 + and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.
AB - The fission yeast Schizosaccharomyces pombe does not grow in media containing glycerol as a sole carbon source but uses glycerol in the presence of ethanol. Ethanol, but not glycerol, triggered upregulation of gld1 + and fbp1 + during glucose starvation even though gld1 + and fbp1 + are essential for growth on glycerol. This upregulation occurred at a very low concentration of ethanol. The transcriptional regulation of gld1 + was tested in the presence of various alcohols, and both ethanol and 1-propanol were found to induce gld1 + and to support growth in glycerol-containing media. We suggest that S. pombe has a novel ethanol and/or 1-propanol recognition mechanism that upregulates glycerol utilization during glucose starvation.
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U2 - 10.1007/s00253-012-3971-x
DO - 10.1007/s00253-012-3971-x
M3 - Article
C2 - 22370953
AN - SCOPUS:84864673073
SN - 0175-7598
VL - 95
SP - 441
EP - 449
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 2
ER -