TY - JOUR
T1 - Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector
AU - Mizuarai, Shinji
AU - Ono, Ken ichiro
AU - Yamaguchi, Kazuhisa
AU - Nishijima, Ken ichi
AU - Kamihira, Masamichi
AU - Iijima, Shinji
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. We thank K. Kanbayashi and T. Horie for technical assistance, and Drs. H. Kagami and H. Iba for providing useful information.
PY - 2001
Y1 - 2001
N2 - We report here the production of transgenic quails using a replication-defective pantropic retroviral vector based on Moloney murine leukemia virus (MoMLV) pseudotyped with vesicular stomatitis virus G protein (VSV-G). The retroviral vector was injected into laid quail embryos at the blastodermal stage, and the embryos were incubated to hatch to produce G0 transgenic quails. Among 134 embryos subjected to viral injection, 37 hatched. The viral vector sequence was detected in the tissues of all Go quails. The germ-line transmission efficiency of Go quails mated with non-transgenic quails was more than 80% on average. Southern blot analysis revealed that the G1 transgenic progeny had one to three copies of the transgene. The expression of vector-encoded neomycin-resistance gene under the control of the Rous sarcoma virus (RSV) promoter was observed in several tissues including heart and muscle of both G1 and G2 transgenic offspring. Due to the high frequency of germ-line transmission, this method may markedly facilitate the production of transgenic avian.
AB - We report here the production of transgenic quails using a replication-defective pantropic retroviral vector based on Moloney murine leukemia virus (MoMLV) pseudotyped with vesicular stomatitis virus G protein (VSV-G). The retroviral vector was injected into laid quail embryos at the blastodermal stage, and the embryos were incubated to hatch to produce G0 transgenic quails. Among 134 embryos subjected to viral injection, 37 hatched. The viral vector sequence was detected in the tissues of all Go quails. The germ-line transmission efficiency of Go quails mated with non-transgenic quails was more than 80% on average. Southern blot analysis revealed that the G1 transgenic progeny had one to three copies of the transgene. The expression of vector-encoded neomycin-resistance gene under the control of the Rous sarcoma virus (RSV) promoter was observed in several tissues including heart and muscle of both G1 and G2 transgenic offspring. Due to the high frequency of germ-line transmission, this method may markedly facilitate the production of transgenic avian.
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U2 - 10.1006/bbrc.2001.5422
DO - 10.1006/bbrc.2001.5422
M3 - Article
C2 - 11511080
AN - SCOPUS:0034815057
SN - 0006-291X
VL - 286
SP - 456
EP - 463
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -