TY - JOUR
T1 - Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium
AU - Inoue, Yuichi
AU - Lopez, Lourdes Bouzo
AU - Kawamoto, Seiji
AU - Arita, Nobuhisa
AU - Teruya, Kiichiro
AU - Seki, Kiyohiko
AU - Shoji, Masahiro
AU - Kamei, Masanori
AU - Hashizume, Shuichi
AU - Shiozawa, Yasumitsu
AU - Tachibana, Hirofumi
AU - Ohashi, Hideya
AU - Yasumoto, Kosei
AU - Nagashima, Akira
AU - Nakahashi, Hisashi
AU - Suzuki, Tatsuo
AU - Imai, Tadaaki
AU - Nomoto, Kikuo
AU - Takenoyama, Mitsuhiro
AU - Katakura, Yoshinori
AU - Shirahata, Sanetaka
PY - 1996/1
Y1 - 1996/1
N2 - A ras oncogene-amplified recombinant BHK-21 cell line (ras-rBHK-IgG) has been established, and was shown to hyperproduce the recombinant IgG chimeric human monoclonal antibody (hMAb) AE6F4, which recognizes lung cancer cells. We found that the ras-rBHK-IgG cells could be easily cultured in a protein-free ERDF medium supplemented with iron(III) nitrate, hydroxyethyliminodiacetic acid, and non-protein synthetic attachment factor as well as in a serum-free ERDF medium supplemented with insulin, transferrin, ethanolamine, and sodium selenite. The productivity of recombinant hMAb from the cells cultured in dishes at high cell densities was higher in protein-free medium than in serum-containing medium.True high density culture of the ras-rBHK-IgG cells was done in protein-free medium using the Tecnomouse, which is a novel hollow fiber bioreactor system. After culture for 30 days in protein-free culture, a total amount of about 14 mg of the recombinant hMAb AE6F4 was obtained, and was shown to be reactive against lung cancer cells in tissues.
AB - A ras oncogene-amplified recombinant BHK-21 cell line (ras-rBHK-IgG) has been established, and was shown to hyperproduce the recombinant IgG chimeric human monoclonal antibody (hMAb) AE6F4, which recognizes lung cancer cells. We found that the ras-rBHK-IgG cells could be easily cultured in a protein-free ERDF medium supplemented with iron(III) nitrate, hydroxyethyliminodiacetic acid, and non-protein synthetic attachment factor as well as in a serum-free ERDF medium supplemented with insulin, transferrin, ethanolamine, and sodium selenite. The productivity of recombinant hMAb from the cells cultured in dishes at high cell densities was higher in protein-free medium than in serum-containing medium.True high density culture of the ras-rBHK-IgG cells was done in protein-free medium using the Tecnomouse, which is a novel hollow fiber bioreactor system. After culture for 30 days in protein-free culture, a total amount of about 14 mg of the recombinant hMAb AE6F4 was obtained, and was shown to be reactive against lung cancer cells in tissues.
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U2 - 10.1271/bbb.60.811
DO - 10.1271/bbb.60.811
M3 - Article
C2 - 8704311
AN - SCOPUS:0030151342
SN - 0916-8451
VL - 60
SP - 811
EP - 817
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -
