Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

K. Honda; H. Shirae; K. Miwa; S. Matsushita; K. Makishima; Y. Nishimura; T. Sasazuki

doi:10.1089/hyb.1992.11.361

Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

K. Honda, H. Shirae, K. Miwa, S. Matsushita, K. Makishima, Y. Nishimura, T. Sasazuki

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG₁) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

Original language	English
Pages (from-to)	361-366
Number of pages	6
Journal	Hybridoma
Volume	11
Issue number	3
DOIs	https://doi.org/10.1089/hyb.1992.11.361
Publication status	Published - 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology
Genetics

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10.1089/hyb.1992.11.361

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title = "Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen",

abstract = "Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.",

author = "K. Honda and H. Shirae and K. Miwa and S. Matsushita and K. Makishima and Y. Nishimura and T. Sasazuki",

year = "1992",

doi = "10.1089/hyb.1992.11.361",

language = "English",

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T1 - Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

AU - Honda, K.

AU - Shirae, H.

AU - Miwa, K.

AU - Matsushita, S.

AU - Makishima, K.

AU - Nishimura, Y.

AU - Sasazuki, T.

PY - 1992

Y1 - 1992

N2 - Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

AB - Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

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JO - Hybridoma

JF - Hybridoma

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ER -

Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

Abstract

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