Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh; Yasuto Hoshikawa; Shun Ichi Matsuura; Junko Mizuguchi; Hiroyuki Arafune; Taka Aki Hanaoka; Fujio Mizukami; Akari Hayashi; Hirotomo Nishihara; Takashi Kyotani

doi:10.1016/j.bej.2012.07.012

Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh, Yasuto Hoshikawa, Shun Ichi Matsuura, Junko Mizuguchi, Hiroyuki Arafune, Taka Aki Hanaoka, Fujio Mizukami, Akari Hayashi, Hirotomo Nishihara, Takashi Kyotani

Advanced devices

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Abstract

A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

Original language	English
Pages (from-to)	207-214
Number of pages	8
Journal	Biochemical Engineering Journal
Volume	68
DOIs	https://doi.org/10.1016/j.bej.2012.07.012
Publication status	Published - Oct 15 2012

All Science Journal Classification (ASJC) codes

Bioengineering
Biotechnology
Environmental Engineering
Biomedical Engineering

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10.1016/j.bej.2012.07.012

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title = "Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability",

abstract = "A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.",

author = "Tetsuji Itoh and Yasuto Hoshikawa and Matsuura, {Shun Ichi} and Junko Mizuguchi and Hiroyuki Arafune and Hanaoka, {Taka Aki} and Fujio Mizukami and Akari Hayashi and Hirotomo Nishihara and Takashi Kyotani",

year = "2012",

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doi = "10.1016/j.bej.2012.07.012",

language = "English",

volume = "68",

pages = "207--214",

journal = "Biochemical Engineering Journal",

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T1 - Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

AU - Itoh, Tetsuji

AU - Hoshikawa, Yasuto

AU - Matsuura, Shun Ichi

AU - Mizuguchi, Junko

AU - Arafune, Hiroyuki

AU - Hanaoka, Taka Aki

AU - Mizukami, Fujio

AU - Hayashi, Akari

AU - Nishihara, Hirotomo

AU - Kyotani, Takashi

PY - 2012/10/15

Y1 - 2012/10/15

N2 - A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

AB - A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

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VL - 68

SP - 207

EP - 214

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Abstract

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