Production of human erythropoietin by chimeric chickens

Daisuke Kodama; Daisuke Nishimiya; Ken ichi Iwata; Kazuhisa Yamaguchi; Kazuhiro Yoshida; Yoshinori Kawabe; Makoto Motono; Hiroyuki Watanabe; Takashi Yamashita; Ken ichi Nishijima; Masamichi Kamihira; Shinji Iijima

doi:10.1016/j.bbrc.2008.01.020

Production of human erythropoietin by chimeric chickens

Daisuke Kodama, Daisuke Nishimiya, Ken ichi Iwata, Kazuhisa Yamaguchi, Kazuhiro Yoshida, Yoshinori Kawabe, Makoto Motono, Hiroyuki Watanabe, Takashi Yamashita, Ken ichi Nishijima, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.

Original language	English
Pages (from-to)	834-839
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	367
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2008.01.020
Publication status	Published - Mar 21 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.01.020

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@article{507aee96f4c54325bd5e739f0ff3209f,

title = "Production of human erythropoietin by chimeric chickens",

abstract = "The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.",

author = "Daisuke Kodama and Daisuke Nishimiya and Iwata, {Ken ichi} and Kazuhisa Yamaguchi and Kazuhiro Yoshida and Yoshinori Kawabe and Makoto Motono and Hiroyuki Watanabe and Takashi Yamashita and Nishijima, {Ken ichi} and Masamichi Kamihira and Shinji Iijima",

note = "Funding Information: This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by the New Energy and Industrial Technology Development Organization (NEDO). The authors are grateful to Radioisotope Center, Nagoya University for supporting the experiments involving radioisotope. ",

year = "2008",

month = mar,

day = "21",

doi = "10.1016/j.bbrc.2008.01.020",

language = "English",

volume = "367",

pages = "834--839",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

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TY - JOUR

T1 - Production of human erythropoietin by chimeric chickens

AU - Kodama, Daisuke

AU - Nishimiya, Daisuke

AU - Iwata, Ken ichi

AU - Yamaguchi, Kazuhisa

AU - Yoshida, Kazuhiro

AU - Kawabe, Yoshinori

AU - Motono, Makoto

AU - Watanabe, Hiroyuki

AU - Yamashita, Takashi

AU - Nishijima, Ken ichi

AU - Kamihira, Masamichi

AU - Iijima, Shinji

N1 - Funding Information: This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by the New Energy and Industrial Technology Development Organization (NEDO). The authors are grateful to Radioisotope Center, Nagoya University for supporting the experiments involving radioisotope.

PY - 2008/3/21

Y1 - 2008/3/21

N2 - The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.

AB - The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.

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U2 - 10.1016/j.bbrc.2008.01.020

DO - 10.1016/j.bbrc.2008.01.020

M3 - Article

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AN - SCOPUS:38649130994

SN - 0006-291X

VL - 367

SP - 834

EP - 839

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Production of human erythropoietin by chimeric chickens

Abstract

All Science Journal Classification (ASJC) codes

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