TY - JOUR
T1 - Production of glycyrrhizin in callus cultures of licorice
AU - Wongwicha, Winida
AU - Tanaka, Hiroyuki
AU - Shoyama, Yukihiro
AU - Tuvshintogtokh, Indree
AU - Putalun, Waraporn
N1 - Funding Information:
This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS), Asian Core Program (Medicinal Plant Breeding Division).
PY - 2008
Y1 - 2008
N2 - Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l α-naphthaleneacetic acid (NAA) and 0.5 mg/l 6-benzyladenine (BA) (67%) which also gave maximum shoot formation per explant (two shoots per explant). These results indicated that all three Glycyrrhiza species regenerated shoots from callus cultures on MS medium combined with NAA and BA or only thidiazuron (TDZ; 0.1 and 0.5 mg/l). Glycyrrhizin contents of G. uralensis calli induced using MS medium in combination with NAA and BA [(27.60 ± 8.47) μg/g DW] or TDZ alone [(36.52 ± 2.45) μg/g DW] were higher than those found in other combinations.
AB - Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l α-naphthaleneacetic acid (NAA) and 0.5 mg/l 6-benzyladenine (BA) (67%) which also gave maximum shoot formation per explant (two shoots per explant). These results indicated that all three Glycyrrhiza species regenerated shoots from callus cultures on MS medium combined with NAA and BA or only thidiazuron (TDZ; 0.1 and 0.5 mg/l). Glycyrrhizin contents of G. uralensis calli induced using MS medium in combination with NAA and BA [(27.60 ± 8.47) μg/g DW] or TDZ alone [(36.52 ± 2.45) μg/g DW] were higher than those found in other combinations.
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U2 - 10.1515/znc-2008-5-617
DO - 10.1515/znc-2008-5-617
M3 - Article
C2 - 18669029
AN - SCOPUS:48249131665
SN - 0939-5075
VL - 63
SP - 413
EP - 417
JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences
IS - 5-6
ER -