TY - JOUR
T1 - Production of chimeric monoclonal antibodies by genetically manipulated chickens
AU - Kamihira, Masamichi
AU - Kawabe, Yoshinori
AU - Shindo, Takuya
AU - Ono, Ken ichiro
AU - Esaka, Kazuhisa
AU - Yamashita, Takashi
AU - Nishijima, Ken ichi
AU - Iijima, Shinji
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research (nos. 14350434 and 20360376) from the Japan Society for the Promotion of Science (JSPS) and Technology Incubation Program in Advanced Region by the Japan Science and Technology Agency (JST).
PY - 2009/4/20
Y1 - 2009/4/20
N2 - Genetically manipulated chickens producing chimeric monoclonal antibodies were generated by injecting retroviral vectors encoding genes for the heavy and light chains of antibodies into developing embryos. The transgene was detected in all chickens that hatched, and they stably produced the chimeric antibodies in their serum. After sexual maturation, the antibodies were also produced in eggs laid by the manipulated hens. The stable antibody production was observed both in egg white and yolk throughout the breeding period. The chimeric antibodies produced by the chickens were properly assembled and exhibited antigen-binding activities. Furthermore, we characterized the structures of the N-linked oligosaccharide chains added to the Fc-region of the recombinant antibodies produced in the serum, egg white and yolk of the chickens.
AB - Genetically manipulated chickens producing chimeric monoclonal antibodies were generated by injecting retroviral vectors encoding genes for the heavy and light chains of antibodies into developing embryos. The transgene was detected in all chickens that hatched, and they stably produced the chimeric antibodies in their serum. After sexual maturation, the antibodies were also produced in eggs laid by the manipulated hens. The stable antibody production was observed both in egg white and yolk throughout the breeding period. The chimeric antibodies produced by the chickens were properly assembled and exhibited antigen-binding activities. Furthermore, we characterized the structures of the N-linked oligosaccharide chains added to the Fc-region of the recombinant antibodies produced in the serum, egg white and yolk of the chickens.
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U2 - 10.1016/j.jbiotec.2009.02.022
DO - 10.1016/j.jbiotec.2009.02.022
M3 - Article
C2 - 19428726
AN - SCOPUS:64249173171
SN - 0168-1656
VL - 141
SP - 18
EP - 25
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 1-2
ER -