Production and characterization of highly specific monoclonal antibodies to D-glutamic acid

Seiichi Sakamoto, Yurino Matsuura, Yayoi Yonenaga, Yumi Tsuneura, Mariko Aso, Hitoshi Kurose, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Most of the functions of D-amino acids (D-AA) remain unclear because of little analytic methods for specific detection/determination. In this study, a highly specific monoclonal antibody to D-glutamic acid (D-Glu-MAb) was produced using a hybridoma method. Characterization of D-Glu-MAb by indirect enzyme-linked immunosorbent assay (ELISA) revealed that it has high selectivity against D-Glu-glutaraldehyde (GA) conjugates, while no cross-reaction was observed when 38 other kinds of AA-GA conjugates were used. Moreover, subsequent indirect competitive ELISA disclosed that an epitope of D-Glu-MAb is a D-Glu-GA molecule in the conjugates, suggesting that D-Glu-MAb could be a useful tool to investigate the functional analysis of D-Glu in immunostaining.

Original languageEnglish
Pages (from-to)414-419
Number of pages6
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Issue number6
Publication statusPublished - Dec 1 2014

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Production and characterization of highly specific monoclonal antibodies to D-glutamic acid'. Together they form a unique fingerprint.

Cite this