TY - JOUR
T1 - Primary Structure of a Cysteine Proteinase Inhibitor from the Fruit of Avocado (Persea americana Mill)
AU - Kimura, Makoto
AU - Yamasaki, Nobuyuki
AU - Ikeda, Tomoko
AU - Fukumoto, Daisuke
AU - Yonekura, Masami
N1 - Funding Information:
Acknowledgments. We thank Dr. Wataru Kugimiya of the Central Research Institute, Tsukuba R&D Center, Fuji Oil Co., Ltd., for a direct N-terminal sequencing of avocado cystatin. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (No. 05258203) from the Ministry of Education, Science, and Culture of Japan.
PY - 1995
Y1 - 1995
N2 - The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin)is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11, 300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.
AB - The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin)is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11, 300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.
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U2 - 10.1271/bbb.59.2328
DO - 10.1271/bbb.59.2328
M3 - Article
C2 - 8611758
AN - SCOPUS:0029447431
SN - 0916-8451
VL - 59
SP - 2328
EP - 2329
JO - Bioscience, biotechnology, and biochemistry
JF - Bioscience, biotechnology, and biochemistry
IS - 12
ER -