Primary culture of rat hepatocytes using membrane-supported collagen sandwich with hormone-free medium

Kazuhiro Ichikawa, Yasuyuki Sakai, Akiyoshi Sakoda, Motoyuki Suzuki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Primary culture of rat hepatocytes in hormone-free medium using membrane-supported collagen sandwich maintained their cellular morphology and expressed albumin secretion for about 3 weeks in vitro. It was reconfirmed that mimicking the cellular environment in vivo was effective for cellular maintenance.

Original languageEnglish
Pages (from-to)385-388
Number of pages4
JournalBiotechnology Techniques
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Applied Microbiology and Biotechnology

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