TY - JOUR
T1 - Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model
AU - Fukuhira, Yukako
AU - Ito, Masaya
AU - Kaneko, Hiroaki
AU - Sumi, Yoshihiko
AU - Tanaka, Masaru
AU - Yamamoto, Sadaaki
AU - Shimomura, Masatsugu
PY - 2008/8
Y1 - 2008/8
N2 - Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and ω-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films on the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.1 ± 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 ± 0.8 g, p < 0.05). Further, the adhesion score after 1 week for the HCPLA-DOPE group (1.6 ± 0.2) was significantly lower than that for the control group (3.0 ±0.3, ñ< 0.05) but comparable to that for the Seprafilm™ group (1.4 ± 0.3). These results demonstrated the potential of HCPLA-DOPE as a physical barrier for preventing postoperative adhesion.
AB - Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and ω-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films on the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.1 ± 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 ± 0.8 g, p < 0.05). Further, the adhesion score after 1 week for the HCPLA-DOPE group (1.6 ± 0.2) was significantly lower than that for the control group (3.0 ±0.3, ñ< 0.05) but comparable to that for the Seprafilm™ group (1.4 ± 0.3). These results demonstrated the potential of HCPLA-DOPE as a physical barrier for preventing postoperative adhesion.
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U2 - 10.1002/jbm.b.31026
DO - 10.1002/jbm.b.31026
M3 - Article
C2 - 18161833
AN - SCOPUS:47949086076
SN - 1552-4973
VL - 86
SP - 353
EP - 359
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 2
ER -