TY - GEN
T1 - Prevention of nuclear mixing of fused cells interconnected through a microslit using rock inhibitor y-27632 for direct cytoplasmic transfer between live single cells
AU - Wada, K. I.
AU - Hosokawa, K.
AU - Ito, Y.
AU - Maeda, M.
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - We previously reported a cell fusion method using a microfluidic device, in which paired single cells were fused through a microslit [1]. Although our approach realized direct cytoplasmic transfer between live single cells and thus is a promising method to induce cellular reprogramming or transdifferentiation, prevention of nuclear mixing with the microslit was unsatisfactory (∼15%). We herein report that the nuclear mixing was almost perfectly prevented (∼95%) by addition of ROCK inhibitor Y-27632 in NIH3T3 fibroblast. Furthermore, Y-27632 raised the probability of cell fusion through the microslit. As a result, we obtained 16.5-fold increase in the number of nuclear-separated fused cell pairs.
AB - We previously reported a cell fusion method using a microfluidic device, in which paired single cells were fused through a microslit [1]. Although our approach realized direct cytoplasmic transfer between live single cells and thus is a promising method to induce cellular reprogramming or transdifferentiation, prevention of nuclear mixing with the microslit was unsatisfactory (∼15%). We herein report that the nuclear mixing was almost perfectly prevented (∼95%) by addition of ROCK inhibitor Y-27632 in NIH3T3 fibroblast. Furthermore, Y-27632 raised the probability of cell fusion through the microslit. As a result, we obtained 16.5-fold increase in the number of nuclear-separated fused cell pairs.
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M3 - Conference contribution
AN - SCOPUS:84983346307
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 792
EP - 794
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -