Presence of non-selective type of endothelin receptor on vascular endothelium and its linkage to vasodilation

Ryoichi Takayanagi; Kazuhiro Kitazumi; Chikahisa Takasaki; Keizo Ohnaka; Saburo Aimoto; Kenjí Tasaka; Masao Ohashi; Hajime Nawata

doi:10.1016/0014-5793(91)80454-B

Presence of non-selective type of endothelin receptor on vascular endothelium and its linkage to vasodilation

Ryoichi Takayanagi, Kazuhiro Kitazumi, Chikahisa Takasaki, Keizo Ohnaka, Saburo Aimoto, Kenjí Tasaka, Masao Ohashi, Hajime Nawata

Research output: Contribution to journal › Article › peer-review

236 Citations (Scopus)

Abstract

We studied the role of non-selective type (ET₁₄) of endothelin (ET) receptor in the vasculature, using a ligand specific to the ET₁₄ receptor, [Glu⁹]-sarafotoxin S6b ([Glu⁹]SRTb). Endothelium-containing rat thoracic aorta possessed specific binding sites for ¹²⁵I-[Glu⁹]SRTb, which were almost eliminated by removal of the endothelium, while ET-3-specific binding sites were not detected in the endothelium-intact rat aorta. Only ET₁₄ receptor was detected in the membranes from the endothelium of porcine thoracic aorta. [Glu⁹]SRTb exerted only vasodilation in rat aortic ring. These findings indicate that ET₁₄ receptors are located on vascular endothelium and linked to vasodilation.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	FEBS Letters
Volume	282
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(91)80454-B
Publication status	Published - Apr 22 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80454-B

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abstract = "We studied the role of non-selective type (ET14) of endothelin (ET) receptor in the vasculature, using a ligand specific to the ET14 receptor, [Glu9]-sarafotoxin S6b ([Glu9]SRTb). Endothelium-containing rat thoracic aorta possessed specific binding sites for 125I-[Glu9]SRTb, which were almost eliminated by removal of the endothelium, while ET-3-specific binding sites were not detected in the endothelium-intact rat aorta. Only ET14 receptor was detected in the membranes from the endothelium of porcine thoracic aorta. [Glu9]SRTb exerted only vasodilation in rat aortic ring. These findings indicate that ET14 receptors are located on vascular endothelium and linked to vasodilation.",

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AU - Takayanagi, Ryoichi

AU - Kitazumi, Kazuhiro

AU - Takasaki, Chikahisa

AU - Ohnaka, Keizo

AU - Aimoto, Saburo

AU - Tasaka, Kenjí

AU - Ohashi, Masao

AU - Nawata, Hajime

PY - 1991/4/22

Y1 - 1991/4/22

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AB - We studied the role of non-selective type (ET14) of endothelin (ET) receptor in the vasculature, using a ligand specific to the ET14 receptor, [Glu9]-sarafotoxin S6b ([Glu9]SRTb). Endothelium-containing rat thoracic aorta possessed specific binding sites for 125I-[Glu9]SRTb, which were almost eliminated by removal of the endothelium, while ET-3-specific binding sites were not detected in the endothelium-intact rat aorta. Only ET14 receptor was detected in the membranes from the endothelium of porcine thoracic aorta. [Glu9]SRTb exerted only vasodilation in rat aortic ring. These findings indicate that ET14 receptors are located on vascular endothelium and linked to vasodilation.

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Presence of non-selective type of endothelin receptor on vascular endothelium and its linkage to vasodilation

Abstract

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