Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

Akihiro Sakoguchi; Sachiko Handa; Fumiyuki Nakashio; Shin Ya Okazaki; Masahiro Goto

doi:10.1252/kakoronbunshu.27.57

Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

Akihiro Sakoguchi, Sachiko Handa, Fumiyuki Nakashio, Shin Ya Okazaki, Masahiro Goto

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.

Original language	English
Pages (from-to)	61-62
Number of pages	2
Journal	kagaku kogaku ronbunshu
Volume	27
Issue number	1
DOIs	https://doi.org/10.1252/kakoronbunshu.27.57
Publication status	Published - 2001

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.1252/kakoronbunshu.27.57

Cite this

@article{3d84b44fdc9a4db7a792b0a718b6ce68,

title = "Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents",

abstract = "The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.",

author = "Akihiro Sakoguchi and Sachiko Handa and Fumiyuki Nakashio and Okazaki, {Shin Ya} and Masahiro Goto",

year = "2001",

doi = "10.1252/kakoronbunshu.27.57",

language = "English",

volume = "27",

pages = "61--62",

journal = "kagaku kogaku ronbunshu",

issn = "0386-216X",

publisher = "The Society of Chemical Engineers, Japan",

number = "1",

}

TY - JOUR

T1 - Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

AU - Sakoguchi, Akihiro

AU - Handa, Sachiko

AU - Nakashio, Fumiyuki

AU - Okazaki, Shin Ya

AU - Goto, Masahiro

PY - 2001

Y1 - 2001

N2 - The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.

AB - The characteristics of the surfactant-subtilisin Carlsberg (STC) complex are investigated on an enzymatic ester-exchange reaction in an organic solvent. The surfactant-STC complex exhibits a high enzymatic activity in various organic solvents although native STC hardly catalyzes the same reaction. The results reveal that the pH value and the surfactant concentration at the preparation stage have a prominent effect to ensure high catalytic activity of the enzyme complex in organic media. The high solubility of the surfactant-STC complex in organic media makes it possible to obtain direct information about the reaction mechanism by kinetic studies.

UR - http://www.scopus.com/inward/record.url?scp=23044523429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23044523429&partnerID=8YFLogxK

U2 - 10.1252/kakoronbunshu.27.57

DO - 10.1252/kakoronbunshu.27.57

M3 - Article

AN - SCOPUS:23044523429

SN - 0386-216X

VL - 27

SP - 61

EP - 62

JO - kagaku kogaku ronbunshu

JF - kagaku kogaku ronbunshu

IS - 1

ER -

Preparation of surfactant-subtilisin carlsberg complexes catalytically active in organic solvents

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this