Preparation of surfactant-enzyme complex utilizing water-in-oil emulsion

Shin Ya Okazaki, Kojiro Abe, Masahiro Goto, Fumiyuki Nakashio

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)


    A novel preparation method for surfactant-enzyme complexes applicable in organic media is developed utilizing a water-in-oil (W/O) emulsion. This preparation method entails simple stages while it offers the advantages of enhanced enzymatic activity in the reacting media and eliminates enzyme loss. Using the surfactant-chymotrypsin complex, peptide synthesis with amino acid derivatives was conducted in tetrahydrofuran. The surfactant-chymotrypsin complex indicates higher activity than native chymotrypsin. This modified method will facilitate the usage of enzymes in organic media as new biocatalysts.

    Original languageEnglish
    Number of pages1
    JournalKagaku Kogaku Ronbunshu
    Issue number4
    Publication statusPublished - Dec 1 1997

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)


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