TY - JOUR
T1 - Preparation of protoplasts from chiorella ellipsoidea C-27
AU - Hatano, Shoji
AU - Joh, Toshio
AU - Miyamoto, Takahisa
AU - Yoshimoto, Makoto
N1 - Funding Information:
The present research was supported by the Egashira Foundation for Promotion of the Hotel and the Restaurant Industry. We are grateful to K I Chemical Industry Co., Ltd. for the gift of Chitosanase KI and to Mr Kenya Ohkushi, Miss Miho Yasukawa and Miss Yumi Choh for technical support.
PY - 1992/7
Y1 - 1992/7
N2 - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.
AB - Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.
UR - http://www.scopus.com/inward/record.url?scp=0009650078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0009650078&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0009650078
SN - 0032-0781
VL - 33
SP - 651
EP - 655
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 5
ER -