Preparation of protoplasts from chiorella ellipsoidea C-27

Shoji Hatano, Toshio Joh, Takahisa Miyamoto, Makoto Yoshimoto

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell homogenate of C-27 cells. The formation of naked protoplasts was demonstrated by electron microscopy.

Original languageEnglish
Pages (from-to)651-655
Number of pages5
JournalPlant and Cell Physiology
Issue number5
Publication statusPublished - Jul 1992

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology


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