Preparation of homogenous oligosaccharide chains from glycosphingolipids

Yu Teh Li; Chau Wen Chou; Su Chen Li; Utaro Kobayashi; Yo Hey Ishibashi; Makoto Ito

doi:10.1007/s10719-008-9125-9

Preparation of homogenous oligosaccharide chains from glycosphingolipids

Yu Teh Li, Chau Wen Chou, Su Chen Li, Utaro Kobayashi, Yo Hey Ishibashi, Makoto Ito

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II³NeuAcGgOse₄Cer) and GbOse₄Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

Original language	English
Pages (from-to)	929-933
Number of pages	5
Journal	Glycoconjugate Journal
Volume	26
Issue number	8
DOIs	https://doi.org/10.1007/s10719-008-9125-9
Publication status	Published - Nov 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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title = "Preparation of homogenous oligosaccharide chains from glycosphingolipids",

abstract = "After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.",

author = "Li, {Yu Teh} and Chou, {Chau Wen} and Li, {Su Chen} and Utaro Kobayashi and Ishibashi, {Yo Hey} and Makoto Ito",

note = "Funding Information: Acknowledgements This work was supported by NIH Grant Number R01NS009626 from the National Institute of Neurological Disorders and stroke (to Y.T.L) and Basic Research B 1938006 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (to M.I).",

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doi = "10.1007/s10719-008-9125-9",

language = "English",

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AU - Li, Yu Teh

AU - Chou, Chau Wen

AU - Li, Su Chen

AU - Kobayashi, Utaro

AU - Ishibashi, Yo Hey

AU - Ito, Makoto

N1 - Funding Information: Acknowledgements This work was supported by NIH Grant Number R01NS009626 from the National Institute of Neurological Disorders and stroke (to Y.T.L) and Basic Research B 1938006 from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government (to M.I).

PY - 2009/11

Y1 - 2009/11

N2 - After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

AB - After the discovery of glycosphingolipid (GSL) glycan detaching enzymes, Rhodococcal endoglycoceramidase (EGCase) and leech ceramide glycanase (CGase), the method for enzymatically releasing glycans from GSLs has become the method of choice for preparing intact ceramide-free oligosaccharide chains from GSLs. This paper describes (1) the preparation of the intact oligosaccharides from GM1 (II3NeuAcGgOse4Cer) and GbOse4Cer as examples to show the use of CGase to prepare intact glycan chains from GSLs, and (2) the specificity and detergent requirements of Rhodococcal EGCases for the release of glycan chains from different GSLs.

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ER -

Preparation of homogenous oligosaccharide chains from glycosphingolipids

Abstract

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