Preparation of glycopolymer-substituted gold nanoparticles and their molecular recognition

Glycopolymer-substituted gold nanoparticles were prepared via living radical polymerization with a reversible addition-fragmentation chain transfer (RAFT) reagent. Polyacrylamide derivatives with α-mannose (α-Man) and AT-acetyl-/?glucosamine (β-GlcNAc) were synthesized and hydrogenated to obtain thiol-terminated polymer. The thiol-terminated glycopolymers were mixed with gold nanoparticles to yield the polymer substituted gold nanoparticles with various diameters, which aggregated on addition of saccharide-recognition proteins (lectins). The aggregation properties were analyzed using transmission electron microscopy and UV spectra. Molecular recognition was studied with E. coli, which induced aggregation of the nanoparticles at the cell periphery.