Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells

Takuya Sugahara; Shinichi Kawashima; Akiko Oda; Yoshio Hisaeda; Keiichi Kato

doi:10.1007/s10616-005-3764-5

Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells

Takuya Sugahara, Shinichi Kawashima, Akiko Oda, Yoshio Hisaeda, Keiichi Kato

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.

Original language	English
Pages (from-to)	51-57
Number of pages	7
Journal	Cytotechnology
Volume	47
Issue number	1-3
DOIs	https://doi.org/10.1007/s10616-005-3764-5
Publication status	Published - Jan 2005

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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abstract = "The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.",

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T1 - Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells

AU - Sugahara, Takuya

AU - Kawashima, Shinichi

AU - Oda, Akiko

AU - Hisaeda, Yoshio

AU - Kato, Keiichi

PY - 2005/1

Y1 - 2005/1

N2 - The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.

AB - The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.

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Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells

Abstract

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