Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…

Yoko Shoji; Ayako Sanekata; Masao Sato; Katsumi Imaizumi

doi:10.1271/bbb.67.1177

Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…

Yoko Shoji, Ayako Sanekata, Masao Sato, Katsumi Imaizumi

Food Science and Biotechnology

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Abstract

Anti-rat Δ6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat Δ6-desaturase. The antiserum prevented the enzymatic activity of Δ6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor α, is involved in activating Δ6-desaturase gene expression, thereby elevating the protein level and the activity.

Original language	English
Pages (from-to)	1177-1178
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	5
DOIs	https://doi.org/10.1271/bbb.67.1177
Publication status	Published - Jan 1 2003

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1271/bbb.67.1177

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Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…. / Shoji, Yoko; Sanekata, Ayako; Sato, Masao et al.
In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 5, 01.01.2003, p. 1177-1178.

Research output: Contribution to journal › Article › peer-review

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Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…

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