TY - JOUR
T1 - Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies
AU - Tanaka, Hiroyuki
AU - Yan, Shayu
AU - Miura, Norio
AU - Shoyama, Yukihiro
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Uehara Memorial Foundation. Support for this work by New Energy and Industrial Technology Development Organization (NEDO) is greatly acknowledged.
PY - 2003
Y1 - 2003
N2 - The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2-3 μg ml-1 for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 μg ml -1.
AB - The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2-3 μg ml-1 for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 μg ml -1.
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U2 - 10.1023/B:CYTO.0000009919.62901.27
DO - 10.1023/B:CYTO.0000009919.62901.27
M3 - Article
C2 - 19002932
AN - SCOPUS:1642419547
SN - 0920-9069
VL - 42
SP - 101
EP - 107
JO - Cytotechnology
JF - Cytotechnology
IS - 2
ER -