TY - JOUR
T1 - Preparation and characterization of a VEGF-Fc fusion protein matrix for enhancing HUVEC growth
AU - Yu, Meihua
AU - Du, Fengyi
AU - Ise, Hirohiko
AU - Zhao, Wei
AU - Zhang, Yan
AU - Yu, Yaoting
AU - Yao, Fanglian
AU - Yang, Jun
AU - Akaike, Toshihiro
N1 - Funding Information:
Acknowledgments This study was supported by the National Natural Science Foundation of China (Grant Nos. 31070855, 51073119), the 973 Program (Grant No. 2011CB606202) and the Natural Science Foundation of Tianjin (Grant No. 10JCYBJC10400).
PY - 2012/10
Y1 - 2012/10
N2 - To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.
AB - To enhance vascularization of hydrophobic implants in vivo, a VEGF-Fc fusion protein consisting of vascular endothelial growth factor (VEGF) fused to the immunoglobulin G Fc domain was prepared as an artificial extracellular matrix (ECM). VEGF-Fc was stably immobilized on a polystyrene plate due to the hydrophobicity of the Fc domain, and significantly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). Additionally, the use of VEGF-Fc as an ECM markedly promoted the proliferation of HUVECs longer than 72 h and induced the reorganization of actin filaments into larger stress fibers within these cells. The VEGF-Fc fusion protein may be a promising artificial ECM for enhancing endothelial cell growth.
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U2 - 10.1007/s10529-012-0959-7
DO - 10.1007/s10529-012-0959-7
M3 - Article
C2 - 22661013
AN - SCOPUS:84865707054
SN - 0141-5492
VL - 34
SP - 1765
EP - 1771
JO - Biotechnology letters
JF - Biotechnology letters
IS - 9
ER -