TY - JOUR
T1 - Possible function of GDNF and Schwann cells in wound healing of periodontal tissue
AU - Itoyama, Tomohiro
AU - Yoshida, Shinichiro
AU - Tomokiyo, Atsushi
AU - Hasegawa, Daigaku
AU - Hamano, Sayuri
AU - Sugii, Hideki
AU - Ono, Taiga
AU - Fujino, Shoko
AU - Maeda, Hidefumi
N1 - Funding Information:
This study was financially supported by Grants‐in‐Aid for Scientific Research (Project Nos. JP17H01598, JP17H04385, JP18K19651, JP19K19001, JP19K19002, and JP19K19031) from the Japan Society for the Promotion of Science. We appreciate Drs. Ipposhi, Kaneko, Albougha, and Yamashita for their assistance in carrying out this work. We also thank Julia Jenkins, PhD, from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2020/12
Y1 - 2020/12
N2 - Objective: The purpose of this study was to evaluate the function of Schwann cells in wound healing of periodontal tissue. Background: In our previous study, glial cell line-derived neurotrophic factor (GDNF) promoted the migration of human periodontal ligament (PDL) cells and that GDNF expression increased in wounded periodontal tissue. GDNF reportedly induces the migration of Schwann cell precursors. Schwann cells play a crucial role in the regeneration of peripheral tissues, including bone tissue. However, the role of Schwann cells on periodontal tissue regeneration remains unclear. Methods: A transwell assay and a WST-1 (water-soluble tetrazolium compound-1) proliferation assay were used to determine whether GDNF promotes the migration and proliferation of Schwann cells, respectively. Quantitative RT-PCR and Alizarin Red S staining were performed to examine the effect of these cells on the differentiation of human preosteoblast (Saos2 cells) using conditioned medium from YST-1 (YST-1-CM). Western blotting analysis was performed to determine whether YST-1-CM activates ERK signaling pathway in Saos2 cells. The expression of Schwann cell markers, S100 calcium-binding protein B (S100-B) and growth associated protein 43 (GAP-43), was determined in normal and wounded periodontal tissue by immunofluorescent staining. Results: Glial cell line-derived neurotrophic factor promoted the migration of YST-1 cells but did not affect the proliferation of YST-1 cells. Saos2 cells cultured with YST-1-CM increased the expression of osteoblastic markers and mineralization. YST-1-CM also induced phosphorylation of ERK1/2 in Saos2 cells. The number of S100-B-immunoreactive cells which also expressed GAP-43 was increased in rat wounded periodontal tissue during healing process. Conclusion: The accumulation of Schwann cells in wounded periodontal tissue suggests that they play a significant role in wound healing of this tissue, especially alveolar bone tissue.
AB - Objective: The purpose of this study was to evaluate the function of Schwann cells in wound healing of periodontal tissue. Background: In our previous study, glial cell line-derived neurotrophic factor (GDNF) promoted the migration of human periodontal ligament (PDL) cells and that GDNF expression increased in wounded periodontal tissue. GDNF reportedly induces the migration of Schwann cell precursors. Schwann cells play a crucial role in the regeneration of peripheral tissues, including bone tissue. However, the role of Schwann cells on periodontal tissue regeneration remains unclear. Methods: A transwell assay and a WST-1 (water-soluble tetrazolium compound-1) proliferation assay were used to determine whether GDNF promotes the migration and proliferation of Schwann cells, respectively. Quantitative RT-PCR and Alizarin Red S staining were performed to examine the effect of these cells on the differentiation of human preosteoblast (Saos2 cells) using conditioned medium from YST-1 (YST-1-CM). Western blotting analysis was performed to determine whether YST-1-CM activates ERK signaling pathway in Saos2 cells. The expression of Schwann cell markers, S100 calcium-binding protein B (S100-B) and growth associated protein 43 (GAP-43), was determined in normal and wounded periodontal tissue by immunofluorescent staining. Results: Glial cell line-derived neurotrophic factor promoted the migration of YST-1 cells but did not affect the proliferation of YST-1 cells. Saos2 cells cultured with YST-1-CM increased the expression of osteoblastic markers and mineralization. YST-1-CM also induced phosphorylation of ERK1/2 in Saos2 cells. The number of S100-B-immunoreactive cells which also expressed GAP-43 was increased in rat wounded periodontal tissue during healing process. Conclusion: The accumulation of Schwann cells in wounded periodontal tissue suggests that they play a significant role in wound healing of this tissue, especially alveolar bone tissue.
UR - http://www.scopus.com/inward/record.url?scp=85087296184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087296184&partnerID=8YFLogxK
U2 - 10.1111/jre.12774
DO - 10.1111/jre.12774
M3 - Article
C2 - 32562261
AN - SCOPUS:85087296184
SN - 0022-3484
VL - 55
SP - 830
EP - 839
JO - Journal of Periodontal Research
JF - Journal of Periodontal Research
IS - 6
ER -