Possible binding sites for inositol 1,4,5-trisphosphate in macrophages

Toyohiro Ishimatsu; Yuichi Kimura; Tetsuro Ikebe; Koji Yamaguchi; Toshitaka Koga; Masato Hirata

doi:10.1016/S0006-291X(88)81263-4

Possible binding sites for inositol 1,4,5-trisphosphate in macrophages

Toyohiro Ishimatsu, Yuichi Kimura, Tetsuro Ikebe, Koji Yamaguchi, Toshitaka Koga, Masato Hirata

Oral Biological Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We reported that an arylazide derivative of inositol 1,4,5-trisphosphate (InsP₃) caused irreversible InsP₃-induced Ca²⁺ release from saponin-permeabilized macrophages after photoirradiation(1). To determine the specific receptors for InsP₃, presumably present on the endoplastic reticulum, we synthesized isotope-labeled arylazide derivatives of InsP₃; InsP₃ was coupled with p-azidobenzoyl [³h]β -alanine InsP₃-[³h]ABβ A ) or p-[¹²⁵i]azidosalicyl β-alanine (InsP₃-[¹²⁵I]ASβ A). We report here that three proteins may be associated with the Ca²⁺ releasing mechanism, in photoirradiated saponin-permeabilized macrophages and in the microsomal fraction.

Original language	English
Pages (from-to)	1173-1180
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	155
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(88)81263-4
Publication status	Published - Sept 30 1988

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(88)81263-4

Cite this

@article{84188e84b66e4fb59f6ad8e2ce8f531d,

title = "Possible binding sites for inositol 1,4,5-trisphosphate in macrophages",

abstract = "We reported that an arylazide derivative of inositol 1,4,5-trisphosphate (InsP3) caused irreversible InsP3-induced Ca2+ release from saponin-permeabilized macrophages after photoirradiation(1). To determine the specific receptors for InsP3, presumably present on the endoplastic reticulum, we synthesized isotope-labeled arylazide derivatives of InsP3; InsP3 was coupled with p-azidobenzoyl [3h]β -alanine InsP3-[3h]ABβ A ) or p-[125i]azidosalicyl β-alanine (InsP3-[125I]ASβ A). We report here that three proteins may be associated with the Ca2+ releasing mechanism, in photoirradiated saponin-permeabilized macrophages and in the microsomal fraction.",

author = "Toyohiro Ishimatsu and Yuichi Kimura and Tetsuro Ikebe and Koji Yamaguchi and Toshitaka Koga and Masato Hirata",

note = "Funding Information: This study was supported in part by the Naito Foundation and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Aoyagi (Department of Chemistry, Faculty of Science, Kyushu University) for suggestions in preparing the N-hydroxysuccinimide ester of p-azidobenzoic acid, AB E A and ASB A.",

year = "1988",

month = sep,

day = "30",

doi = "10.1016/S0006-291X(88)81263-4",

language = "English",

volume = "155",

pages = "1173--1180",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Possible binding sites for inositol 1,4,5-trisphosphate in macrophages

AU - Ishimatsu, Toyohiro

AU - Kimura, Yuichi

AU - Ikebe, Tetsuro

AU - Yamaguchi, Koji

AU - Koga, Toshitaka

AU - Hirata, Masato

N1 - Funding Information: This study was supported in part by the Naito Foundation and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan. We thank Dr. H. Aoyagi (Department of Chemistry, Faculty of Science, Kyushu University) for suggestions in preparing the N-hydroxysuccinimide ester of p-azidobenzoic acid, AB E A and ASB A.

PY - 1988/9/30

Y1 - 1988/9/30

N2 - We reported that an arylazide derivative of inositol 1,4,5-trisphosphate (InsP3) caused irreversible InsP3-induced Ca2+ release from saponin-permeabilized macrophages after photoirradiation(1). To determine the specific receptors for InsP3, presumably present on the endoplastic reticulum, we synthesized isotope-labeled arylazide derivatives of InsP3; InsP3 was coupled with p-azidobenzoyl [3h]β -alanine InsP3-[3h]ABβ A ) or p-[125i]azidosalicyl β-alanine (InsP3-[125I]ASβ A). We report here that three proteins may be associated with the Ca2+ releasing mechanism, in photoirradiated saponin-permeabilized macrophages and in the microsomal fraction.

AB - We reported that an arylazide derivative of inositol 1,4,5-trisphosphate (InsP3) caused irreversible InsP3-induced Ca2+ release from saponin-permeabilized macrophages after photoirradiation(1). To determine the specific receptors for InsP3, presumably present on the endoplastic reticulum, we synthesized isotope-labeled arylazide derivatives of InsP3; InsP3 was coupled with p-azidobenzoyl [3h]β -alanine InsP3-[3h]ABβ A ) or p-[125i]azidosalicyl β-alanine (InsP3-[125I]ASβ A). We report here that three proteins may be associated with the Ca2+ releasing mechanism, in photoirradiated saponin-permeabilized macrophages and in the microsomal fraction.

UR - http://www.scopus.com/inward/record.url?scp=0023801696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023801696&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(88)81263-4

DO - 10.1016/S0006-291X(88)81263-4

M3 - Article

C2 - 3263123

AN - SCOPUS:0023801696

SN - 0006-291X

VL - 155

SP - 1173

EP - 1180

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Possible binding sites for inositol 1,4,5-trisphosphate in macrophages

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this