TY - JOUR
T1 - Positive correlation between the density of macrophages and T-cells in undifferentiated sarcoma
AU - Komohara, Yoshihiro
AU - Takeya, Hiroto
AU - Wakigami, Nanako
AU - Kusada, Natsuki
AU - Bekki, Hirofumi
AU - Ishihara, Shin
AU - Takeya, Motohiro
AU - Nakashima, Yasuharu
AU - Oda, Yoshinao
N1 - Funding Information:
Acknowledgements We thank Ms. Ikuko Miyakawa and Mr. Takenobu Nakagawa for their technical assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (nos. 16H05162, 17H04060).
Publisher Copyright:
© 2018, The Japanese Society for Clinical Molecular Morphology.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - Undifferentiated sarcoma (US) is a frequent soft tissue sarcoma. Although the 10-year survival rate is around 60%, advanced US is highly resistant to chemo/radiotherapy. The tumor microenvironment (TME) is closely associated with tumor progression. However, few studies of infiltrated immune cells in US have been published. In this study, we evaluated tumor-associated macrophages (TAMs) and CD8-positive cytotoxic T lymphocytes (CTLs) in 28 cases of US. Iba1, CD163, and CD204 were used as markers for TAMs. The density of CTLs was positively correlated with the density of TAMs. However, a negative correlation was seen between the density of CTLs and the percentage of CD204-positive TAMs. We found no significant association between the density of Iba1-/CD204-/CD8-positive cells and clinicopathological factors. No significant correlation between immune cell infiltration and clinical outcome was observed. Although we found no significant association between immune cells and clinicopathological factors, these findings may provide new insight into the characterization of immune cells in the TME of US.
AB - Undifferentiated sarcoma (US) is a frequent soft tissue sarcoma. Although the 10-year survival rate is around 60%, advanced US is highly resistant to chemo/radiotherapy. The tumor microenvironment (TME) is closely associated with tumor progression. However, few studies of infiltrated immune cells in US have been published. In this study, we evaluated tumor-associated macrophages (TAMs) and CD8-positive cytotoxic T lymphocytes (CTLs) in 28 cases of US. Iba1, CD163, and CD204 were used as markers for TAMs. The density of CTLs was positively correlated with the density of TAMs. However, a negative correlation was seen between the density of CTLs and the percentage of CD204-positive TAMs. We found no significant association between the density of Iba1-/CD204-/CD8-positive cells and clinicopathological factors. No significant correlation between immune cell infiltration and clinical outcome was observed. Although we found no significant association between immune cells and clinicopathological factors, these findings may provide new insight into the characterization of immune cells in the TME of US.
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U2 - 10.1007/s00795-018-0201-3
DO - 10.1007/s00795-018-0201-3
M3 - Article
C2 - 29980952
AN - SCOPUS:85049556022
SN - 1860-1480
VL - 52
SP - 44
EP - 51
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 1
ER -