Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology

Wataru Koto; Yoshinori Shinohara; Kazuyuki Kitamura; Takanori Wachi; Seicho Makihira; Kiyoshi Koyano

doi:10.3390/nano7100322

Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology

Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano

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Abstract

Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial-mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.

Original language	English
Article number	322
Journal	Nanomaterials
Volume	7
Issue number	10
DOIs	https://doi.org/10.3390/nano7100322
Publication status	Published - Oct 13 2017

All Science Journal Classification (ASJC) codes

Chemical Engineering(all)
Materials Science(all)

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10.3390/nano7100322

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title = "Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology",

abstract = "Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial-mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.",

author = "Wataru Koto and Yoshinori Shinohara and Kazuyuki Kitamura and Takanori Wachi and Seicho Makihira and Kiyoshi Koyano",

note = "Funding Information: Acknowledgments: This study was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science to Yoshinori Shinohara (Grant No. 16K11602) and Seicho Makihira (Grant No. 15K11162). We appreciate the technical support from the Research Support Center, Graduate School of Medical Sciences, Kyushu University. We also appreciate the technical support from the Laboratory for technical support, Medical Institute of Bioregulation, Kyushu University. We also thank Alison Sherwin, PhD, from Edanz Group (www.edanzediting.com) for editing a draft of this manuscript. Publisher Copyright: {\textcopyright} 2017 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/nano7100322",

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AU - Koto, Wataru

AU - Shinohara, Yoshinori

AU - Kitamura, Kazuyuki

AU - Wachi, Takanori

AU - Makihira, Seicho

AU - Koyano, Kiyoshi

N1 - Funding Information: Acknowledgments: This study was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science to Yoshinori Shinohara (Grant No. 16K11602) and Seicho Makihira (Grant No. 15K11162). We appreciate the technical support from the Research Support Center, Graduate School of Medical Sciences, Kyushu University. We also appreciate the technical support from the Laboratory for technical support, Medical Institute of Bioregulation, Kyushu University. We also thank Alison Sherwin, PhD, from Edanz Group (www.edanzediting.com) for editing a draft of this manuscript. Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2017/10/13

Y1 - 2017/10/13

N2 - Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial-mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.

AB - Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial-mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.

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Porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology

Abstract

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