Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

Taro Kimura; Kazuya Koumoto; Masami Mizu; Kazuo Sakurai; Seiji Shinkai

doi:10.1246/cl.2002.1240

Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

Taro Kimura, Kazuya Koumoto, Masami Mizu, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.¹ We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

Original language	English
Pages (from-to)	1240-1241
Number of pages	2
Journal	Chemistry Letters
Issue number	12
DOIs	https://doi.org/10.1246/cl.2002.1240
Publication status	Published - Dec 5 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.2002.1240

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abstract = "Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.",

author = "Taro Kimura and Kazuya Koumoto and Masami Mizu and Kazuo Sakurai and Seiji Shinkai",

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T1 - Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

AU - Kimura, Taro

AU - Koumoto, Kazuya

AU - Mizu, Masami

AU - Sakurai, Kazuo

AU - Shinkai, Seiji

PY - 2002/12/5

Y1 - 2002/12/5

N2 - Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

AB - Schizophyllan belongs to the β-1,3-glucan family and can form a complex with particular polynucleotides.1 We applied this complexation to specifically separate polynucleotides by constructing a schizophyllan-appended column. The column trapped poly(C) and poly(A), while it did not show any affinity to poly(G), which is consistent with the experimental results obtained in the homogenous solution. The present results indicate that the s-SPG column can be a new separation device for biomolecules.

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JO - Chemistry Letters

JF - Chemistry Letters

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ER -

Polysaccharide-polynucleotide interaction (XI); novel separation system of RNAs by using schizophyllan-appended column

Abstract

All Science Journal Classification (ASJC) codes

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