Polymorphism of prophenoloxidase in the silkworm, Bombyx mori

Kohji Yamamoto; Hiroshi Fujii; Yutaka Banno; Yoichi Aso; Masatsune Ishiguro

doi:10.5109/4501

Polymorphism of prophenoloxidase in the silkworm, Bombyx mori

Kohji Yamamoto, Hiroshi Fujii, Yutaka Banno, Yoichi Aso, Masatsune Ishiguro

Systems Biology

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Abstract

We reported that the hemolymph of the a80 strain of the silkworm, Bombyx mori, included three isoforms of prophenoloxidase, a proenzyme of phenoloxidase. However, their heterogeneity in strains other that a80 has not been investigated. On polyacrylamide gel electrophoresis followed by activity staining for this protein, we discovered that two pro-PO isoforms were revealed in the larval hemolymph of some strains, two strains have three isoforms in their hemolymph, and only hemolymph of a481 strain contain one isoform among silkworm strains we tested. In this paper, we confirmed the polymorphism of prophenoloxidase.

Original language	English
Pages (from-to)	319-324
Number of pages	6
Journal	Journal of the Faculty of Agriculture, Kyushu University
Volume	47
Issue number	2
DOIs	https://doi.org/10.5109/4501
Publication status	Published - Feb 2003

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science

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abstract = "We reported that the hemolymph of the a80 strain of the silkworm, Bombyx mori, included three isoforms of prophenoloxidase, a proenzyme of phenoloxidase. However, their heterogeneity in strains other that a80 has not been investigated. On polyacrylamide gel electrophoresis followed by activity staining for this protein, we discovered that two pro-PO isoforms were revealed in the larval hemolymph of some strains, two strains have three isoforms in their hemolymph, and only hemolymph of a481 strain contain one isoform among silkworm strains we tested. In this paper, we confirmed the polymorphism of prophenoloxidase.",

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AU - Yamamoto, Kohji

AU - Fujii, Hiroshi

AU - Banno, Yutaka

AU - Aso, Yoichi

AU - Ishiguro, Masatsune

PY - 2003/2

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AB - We reported that the hemolymph of the a80 strain of the silkworm, Bombyx mori, included three isoforms of prophenoloxidase, a proenzyme of phenoloxidase. However, their heterogeneity in strains other that a80 has not been investigated. On polyacrylamide gel electrophoresis followed by activity staining for this protein, we discovered that two pro-PO isoforms were revealed in the larval hemolymph of some strains, two strains have three isoforms in their hemolymph, and only hemolymph of a481 strain contain one isoform among silkworm strains we tested. In this paper, we confirmed the polymorphism of prophenoloxidase.

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Polymorphism of prophenoloxidase in the silkworm, Bombyx mori

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