TY - JOUR
T1 - Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood
AU - Moriyama, Kosei
AU - Ando, Chie
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Okamura, Seiichi
AU - Nakano, Shuji
AU - Takagi, Yasumitsu
AU - Miki, Takeyoshi
AU - Nakashima, Yoshiyuki
AU - Hirakawa, Hideki
PY - 2008/7
Y1 - 2008/7
N2 - Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples,PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
AB - Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples,PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
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U2 - 10.1111/j.1348-0421.2008.00048.x
DO - 10.1111/j.1348-0421.2008.00048.x
M3 - Article
C2 - 18667036
AN - SCOPUS:52049105174
SN - 0385-5600
VL - 52
SP - 375
EP - 382
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 7
ER -