Polymer-modified gold nanoparticles via RAFT polymerization: A detailed study for a biosensing application

Masaki Takara, Masayuki Toyoshima, Hirokazu Seto, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)


Glycopolymers of polyacrylamide derivatives with mannose were prepared via the living radical polymerization of a reversible addition-fragmentation chain transfer reagent. The polymers obtained showed narrow polydispersities. The polymer terminal group was reduced to a thiol, and the resulting polymers were mixed with gold nanoparticles to prepare glycopolymer-substituted gold nanoparticles. The mannose density was adjusted by varying the copolymer preparation and the glycopolymer-polyacrylamide mixture. The colloidal stability of the polymer-coated gold nanoparticles is dependent on the mannose density. Polymer-coated nanoparticles with low mannose densities showed better colloidal stabilities. The molecular recognition abilities of the polymer were investigated using UV-vis spectroscopy. The polymer-coated nanoparticles showed strong protein recognition abilities because of multivalent binding effects. Polymers with high mannose densities showed stronger recognition abilities. The molecular recognition abilities of the glycopolymer-polyacrylamide mixed nanoparticles are dependent on the mannose density. An immunochromatographic assay was performed using the polymer-coated nanoparticles. The color was detected from the gold nanoparticles in the nanoparticle systems with strong molecular recognition and good colloid stability.

Original languageEnglish
Pages (from-to)931-939
Number of pages9
JournalPolymer Chemistry
Issue number3
Publication statusPublished - Feb 7 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Polymers and Plastics
  • Organic Chemistry


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